Anti-HA tag 抗体 [12CA5] (ab1424)
Key features and details
- Mouse monoclonal [12CA5] to HA tag
- Suitable for: ICC/IF, ChIP, IP, WB
- Reacts with: Species independent
- Isotype: IgG2b
製品の概要
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製品名
Anti-HA tag antibody [12CA5]
HA tag 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [12CA5] to HA tag -
由来種
Mouse -
特異性
The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells. The antibody works in ChIP but less well than ab9110 (see ChIP data). -
アプリケーション
適用あり: ICC/IF, ChIP, IP, WBmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide. Derived from the human influenza hemaglutinin protein
Database link: ADG21028 -
ポジティブ・コントロール
- WB: Rab6-HA transfected HEK-293T whole cell lysate. IP: Rab6-HA transfected HEK-293T whole cell lysate. ICC/IF: Baby hamster kidney cell expressing HA-tag CFTR protein. ChIP: Xenopus laevis oocytes injected with mRNA for HA-tagged human BORIS.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 3996 HA-Tag Antibody (Clone 12CA5[2]).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: 1% Dibasic monohydrogen potassium phosphate -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
12CA5 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1424の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (1) |
Use a concentration of 10 µg/ml.
|
ChIP |
Use a concentration of 3 µg/ml.
Use 3 µg per 25 µg chromatin (see figure legend). |
|
IP | (5) |
Use a concentration of 10 µg/ml.
|
WB | (6) |
Use a concentration of 1 µg/ml.
|
特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
ChIP
Use a concentration of 3 µg/ml. Use 3 µg per 25 µg chromatin (see figure legend). |
IP
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. |
ターゲット情報
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関連性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
別名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
画像
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Anti-HA tag antibody [12CA5] (ab1424) at 1/1000 dilution + HEK293T whole cell lysate over-expressing HA-tagged Rab6 at 20 µg
Secondary
HRP-conjugated goat anti-mouse polyclonal IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 seconds
Blocked with 5% milk for 30 min at 25°C -
This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunofluorescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody.
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Recombinant HA-tagged Rab6 was immunoprecipitated from 1 mg transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 2 μL ab1424.
Lane 1: Rab6-HA transfected HEK-293T whole cell extract (Input)
Lane 2: ab1424 IP in Rab6-HA transfected HEK-293T whole cell extract.
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Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 3µg of ab1424 (anti-HA, light blue) and 3µg of ab18337 (anti-BORIS, dark blue), and 20µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (75)
ab1424 は 75 報の論文で使用されています。
- Shen J et al. Deubiquitylating Enzyme OTUB1 Facilitates Neuronal Survival After Intracerebral Hemorrhage Via Inhibiting NF-κB-triggered Apoptotic Cascades. Mol Neurobiol 61:1726-1736 (2024). PubMed: 37775718
- Zhang C et al. Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4. iScience 26:105745 (2023). PubMed: 36590171
- Zhou H et al. KLF14 regulates the growth of hepatocellular carcinoma cells via its modulation of iron homeostasis through the repression of iron-responsive element-binding protein 2. J Exp Clin Cancer Res 42:5 (2023). PubMed: 36600258
- Debaugnies M et al. RHOJ controls EMT-associated resistance to chemotherapy. Nature 616:168-175 (2023). PubMed: 36949199
- Xu Q et al. Platycodin-D exerts its anti-cancer effect by promoting c-Myc protein ubiquitination and degradation in gastric cancer. Front Pharmacol 14:1138658 (2023). PubMed: 36950011