Anti-H2R 抗体 (ab39964)
Key features and details
- Goat polyclonal to H2R
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-H2R antibody
H2R 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to H2R -
由来種
Goat -
アプリケーション
適用あり: Flow Cyt, WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Dog
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免疫原
Synthetic peptide corresponding to Human H2R aa 335-349 (C terminal).
Sequence:QEEKPLKLQVWSGTE
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ポジティブ･コントロール
- WB: Human tonsil tissue lysate; Flow Cyt: HeLa cells ICC/IF: HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39964の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use a concentration of 10 µg/ml.
|
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| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.
A 1 hour primary incubation is recommended for this product. |
|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
Flow Cyt
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. A 1 hour primary incubation is recommended for this product. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3274 Human
- Entrez Gene: 15466 Mouse
- Omim: 142703 Human
- SwissProt: P25021 Human
- SwissProt: P97292 Mouse
- Unigene: 247885 Human
- Unigene: 57243 Mouse
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別名
- Gastric receptor 1 antibody
- Gastric receptor I antibody
- H2R antibody
see all
画像
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Western blot - Anti-H2R antibody (ab39964)Anti-H2R antibody (ab39964) at 1 µg/ml + Human tonsil tissue lysates (35µg protein in RIPA buffer)
Predicted band size: 40 kDaDetected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-H2R antibody (ab39964)Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL). -
Flow Cytometry - Anti-H2R antibody (ab39964)Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab39964 は論文での使用が確認できていません。
