Anti-H-FABP 抗体 (ab45966)

Thank you for contacting us and reporting the difficulties you have been experiencing in using the anti-Cardiac FABP antibody (ab45966).

I am sorry to hear that this antibody has not been proving sensitive enough for you currently. There are a few suggestions I would make in order to improve the results obtained. We use BSA blocking with most of our antibodies and switching from milk to BSA can sometimes lead to dramatic changes in sensitivity, as illustrated with the anti-GAPDH antibody (ab9385):

https://www.abcam.com/ab9385

I would therefore advise using 5% BSA in your blocking (we use 5% BSA in 0.1% TBST for 1 hour at room temperature), as well as in your antibody diluent (again, we use 5% BSA in TBST). I would also suggest, if you are not already doing so, to incubate the primary antibody with the membrane overnight at 4°C as this can improve the strength and specificity of the signal.

Please do let me know if these suggestions do not help to improve the results sufficiently for your experiments requiring the detection of the endogenous protein. If this is the case, I will look into if we have an antibody or lot which may be more sensitive.

I look forward to hearing how you get on. Until then, I wish you all the best with your research.

DISCOUNT CODE: *******
Expiration date*******

I am very pleased to hear you would like to accept our offer and test ab28723 in Zebrafinches. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Zebrafinchesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -80C may be viable for up to 10 years. I hope this information helps. Please contact us with any other questions.