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Anti-H-FABP 抗体 [67D3] (ab16916)

Reviews (1)Q&A (2)References (6)
Immunohistochemistry (Frozen sections) - Anti-H-FABP antibody [67D3] (ab16916)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [67D3] (ab16916)

Key features and details

  • Mouse monoclonal [67D3] to H-FABP
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-H-FABP antibody [67D3]
    H-FABP 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [67D3] to H-FABP

  • 由来種

    Mouse

  • 特異性

    Ab16916 binds to human H-FABP. It shows no binding to human intestinal or liver FABP and slightly cross reacts with B-FABP.

  • アプリケーション

    適用あり: IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Full length native protein (purified) corresponding to Human H-FABP.

  • ポジティブ･コントロール

    • Heart cells, recombinant human H-FABP This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.

  • 特記事項


    This antibody can be used to detect ischemia areas of human heart. It is also useful as marker for brain damage.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab16916の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.

  • 配列類似性

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

  • ドメイン

    Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 422 protein antibody
    • Cardiac FABP antibody
    • Cardiac Fatty Acid Binding Protein antibody
    • FABP 11 antibody
    • FABP 3 antibody
    • FABP11 antibody
    • FABP3 antibody
    • FABPH_HUMAN antibody
    • Fatty acid binding protein 11 antibody
    • Fatty acid binding protein 3 antibody
    • Fatty acid binding protein 3 muscle and heart antibody
    • Fatty acid binding protein 3 muscle and heart mammary derived growth inhibitor antibody
    • Fatty acid binding protein 3 muscle antibody
    • Fatty acid binding protein 3, muscle and heart (mammary derived growth inhibitor) antibody
    • Fatty acid binding protein 3, muscle antibody
    • Fatty acid binding protein heart antibody
    • Fatty acid binding protein, heart antibody
    • Fatty acid binding protein, muscle and heart antibody
    • Fatty acid binding protein, skeletal muscle antibody
    • Fatty acid-binding protein 3 antibody
    • Fatty acid-binding protein antibody
    • H FABP antibody
    • H-FABP antibody
    • heart antibody
    • Heart type fatty acid binding protein antibody
    • Heart-type fatty acid-binding protein antibody
    • M FABP antibody
    • M-FABP antibody
    • Mammary derived growth inhibitor antibody
    • Mammary-derived growth inhibitor antibody
    • MDGI antibody
    • Muscle fatty acid binding protein antibody
    • Muscle fatty acid-binding protein antibody
    • Mylein protein P2 homolog antibody
    • O FABP antibody
    • OTTHUMP00000003898 antibody
    • P2 adopocyte protein antibody
    see all

画像

  • Immunohistochemistry (Frozen sections) - Anti-H-FABP antibody [67D3] (ab16916)
    Immunohistochemistry (Frozen sections) - Anti-H-FABP antibody [67D3] (ab16916)

    Immunohistochemical staining of H-FABP in human skeletal muscle tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [67D3] (ab16916)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [67D3] (ab16916)

    IHC image of H-FABP staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16916, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献 (6)

ab16916 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab16916 は 6 報の論文で使用されています。

  • Belcastro L  et al. Decreased Fatty Acid Transporter FABP1 and Increased Isoprostanes and Neuroprostanes in the Human Term Placenta: Implications for Inflammation and Birth Weight in Maternal Pre-Gestational Obesity. Nutrients 13:N/A (2021). PubMed: 34444927
  • Khan D  et al. SIRT6 transcriptionally regulates fatty acid transport by suppressing PPARγ. Cell Rep 35:109190 (2021). PubMed: 34077730
  • Jones HB  et al. Myocardial Steatosis and Necrosis in Atria and Ventricles of Rats Given Pyruvate Dehydrogenase Kinase Inhibitors. Toxicol Pathol N/A:N/A (2014). PubMed: 24742628
  • Chen HM  et al. Heart-type Fatty Acid binding protein is associated with proteinuria in obesity. PLoS One 7:e45691 (2012). IHC-P, IHC-Fr ; Mouse . PubMed: 23029183
  • Heather LC  et al. Adenosine monophosphate-activated protein kinase activation, substrate transporter translocation, and metabolism in the contracting hyperthyroid rat heart. Endocrinology 151:422-31 (2010). WB ; Rat . PubMed: 19940039
  • Nevo J  et al. Mammary-derived growth inhibitor alters traffic of EGFR and induces a novel form of cetuximab resistance. Clin Cancer Res 15:6570-81 (2009). IHC-P ; Human . PubMed: 19825952

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-H-FABP antibody [67D3]

 Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Heart)
Antigen retrieval step
None
Permeabilization
No
Specification
Heart
Blocking step
Serum free as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 0µg/mL · Temperature: 24°C
Fixative
Formaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 26 2009

Question

Does this cardiac FABP antibody cross react with FABP5 and FABP3 isoforms?

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Answer

Thank you for your recent telephone enquiry. I have received confirmation that this antibody is cross reactive with rat and mouse H-FABP (Isoform FABP3). I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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Question

I would like to ask some questions about Ab16916; 1. Is there any known cross reactivity to other FABP protiens? 2. What dilution is recommended for IHC on formalin-fixed paraffin-embedded sections? 3. The Ab is made using a native protein. Should we expect difficulty with denaturing conditions; considering it is a monoclonal? 4. Is ab18062 (Rabbit polyclonal) an alternative for IHC if this antidody does not work? Thank you,

Read More
Answer

Thank you for your enquiry regarding ab16916. The monoclonal antibody binds to human heart FABP. It shows no binding to human intestinal or liver FABP. Western blot analysis showed that the antibody slightly cross reacts with B-FABP. For immunohistology and Western blotting dilutions to be used depending on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. The dilution 1:10 is without amplification with ABC so you can maybe dilute more by using ABC. The antibody is tested for Western blotting under non-reduced conditions. It is possible that denaturing conditions give difficulties however this is never been tested. We are very sorry but ab18062 is no longer included in our product range, I apologise for the inconvenience, We hope this information will be helpful to you. If you have further questions please do not hesitate to contact us.

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