Anti-GW182 抗体 (ab114857)
Key features and details
- Rabbit polyclonal to GW182
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GW182 antibody -
製品の詳細
Rabbit polyclonal to GW182 -
由来種
Rabbit -
アプリケーション
適用あり: IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 1225-1275 of Human GW182 (NP_055309.2).
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ポジティブ・コントロール
- HeLa whole cell lysate (ab150035)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab114857の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at 10 µg/mg of lysate.
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特記事項 |
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IP
Use at 10 µg/mg of lysate. |
ターゲット情報
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機能
Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the GW182 family.
Contains 1 RRM (RNA recognition motif) domain. -
細胞内局在
Cytoplasm > P-body. Mammalian P-bodies are also known as GW bodies. - Information by UniProt
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参照データベース
- Entrez Gene: 27327 Human
- Entrez Gene: 233833 Mouse
- Entrez Gene: 308971 Rat
- Omim: 610739 Human
- SwissProt: Q8NDV7 Human
- SwissProt: Q3UHK8 Mouse
- Unigene: 655057 Human
- Unigene: 102305 Mouse
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別名
- CAG repeat protein 26 antibody
- CAGH26 antibody
- DKFZp666E117 antibody
see all
画像
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Detection of GW182 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab114857 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-GW182 antibody which recognizes a upstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab114857 は論文での使用が確認できていません。