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リコンビナントRabMAb

Anti-GUCY1B3 抗体 [EPR8822] (ab154841)

Reviews (2) Submit a question References (12)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Anti-GUCY1B3 antibody [EPR8822] (ab154841)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8822] to GUCY1B3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-GUCY1B3 antibody [EPR8822]
    GUCY1B3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR8822] to GUCY1B3

  • 由来種

    Rabbit

  • 特異性

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human GUCY1B3 aa 1-100. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • Recombinant Human GUCY1B3 protein (ab152446) can be used as a positive control in WB. Human placenta lysate; Human fetal brain and fetal lung lysates; Human pancreas tissue; Human thyroid gland carcinoma tissue; Mouse brain; Rat brain; Rat spleen; Human kidney.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR8822

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154841の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P
1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/100 -1/250. 
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P
1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/100 -1/250. 

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling GUCY1B3 with Purified ab154841 at 1:400 dilution (2.54 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    All lanes : Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/5000 dilution (purified)

    Lane 1 : Human fetal lung lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates
    Lane 4 : Rat spleen lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 71 kDa



    Blocking and diluting buffer : 5% NFDM/TBST
  • Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    All lanes : Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/1000 dilution (unpurified)

    Lane 1 : Human placenta lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal lung lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 71 kDa

  • Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    All lanes : Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/2000 dilution (unpurified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat kidney tissue lysate
    Lane 8 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 71 kDa
    Observed band size: 65 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling GUCY1B3 using unpurified ab154841 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid gland carcinoma tissue, labeling GUCY1B3 using unpurified ab154841 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)

    Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using unpurified ab154841 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)

    Immunohistochemical analysis of paraffin embedded Human heart tissue using unpurified ab154841 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)

    Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using unpurified ab154841 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Anti-GUCY1B3 antibody [EPR8822] (ab154841)

参考文献 (12)

ab154841 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab154841 は 12 報の論文で使用されています。

  • He H  et al. Activating NO-sGC crosstalk in the mouse vascular niche promotes vascular integrity and mitigates acute lung injury. J Exp Med 220:N/A (2023). PubMed: 36350314
  • Hu BA  et al. Stimulation of soluble guanylate cyclase by vericiguat reduces skeletal muscle atrophy of mice following chemotherapy. Front Pharmacol 14:1112123 (2023). PubMed: 36744261
  • Karimi Galougahi K  et al. β3 adrenergic agonism: A novel pathway which improves right ventricular-pulmonary arterial hemodynamics in pulmonary arterial hypertension. Physiol Rep 11:e15549 (2023). PubMed: 36597221
  • Galley JC  et al. Angiotensin II augments renal vascular smooth muscle soluble GC expression via an AT1 receptor-forkhead box subclass O transcription factor signalling axis. Br J Pharmacol 179:2490-2504 (2022). PubMed: 33963547
  • Schenk MW  et al. Soluble guanylate cyclase signalling mediates etoposide resistance in progressing small cell lung cancer. Nat Commun 12:6652 (2021). PubMed: 34789728
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry free floating abreview for Anti-Guanylyl Cyclase beta 1 antibody [EPR8822]

 Good
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brainstem, cochlear nuclei)
Specification
brainstem, cochlear nuclei
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Lin Lin

Verified customer

投稿 Mar 31 2017

Western blot abreview for Anti-Guanylyl Cyclase beta 1 antibody [EPR8822]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (lung, liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
lung, liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 07 2016

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