Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-GUCY1B3 抗体 (ab24824)

Reviews (1)Q&A (1)References (8)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody (ab24824)
  • Immunocytochemistry/ Immunofluorescence - Anti-GUCY1B3 antibody (ab24824)

Key features and details

  • Rabbit polyclonal to GUCY1B3
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-GUCY1B3 antibody
    GUCY1B3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to GUCY1B3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog, Pig

  • 免疫原

    Synthetic peptide corresponding to GUCY1B3.

  • ポジティブ･コントロール

    • Brain

  • 特記事項

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab24824の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody (ab24824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody (ab24824)

    Human cerebellum stained with Anti GUCY1B3 ab24824 at 1/100 dilution for 30 min at RT.

  • Immunocytochemistry/ Immunofluorescence - Anti-GUCY1B3 antibody (ab24824)
    Immunocytochemistry/ Immunofluorescence - Anti-GUCY1B3 antibody (ab24824)
    ICC/IF image of ab24824 stained SKNSH cells. The cells were Both and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24824, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献 (8)

ab24824 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab24824 は 8 報の論文で使用されています。

  • de Winde CM  et al. Fibroblastic reticular cell response to dendritic cells requires coordinated activity of podoplanin, CD44 and CD9. J Cell Sci 134:N/A (2021). PubMed: 34184727
  • de Winde CM  et al. Podoplanin drives dedifferentiation and amoeboid invasion of melanoma. iScience 24:102976 (2021). PubMed: 34485858
  • Lukácová N  et al. Is Innervation of the Neuromuscular Junction at the Diaphragm Modulated by sGC/cGMP Signaling? Front Physiol 11:700 (2020). PubMed: 32655417
  • Waddingham MT  et al. Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model. J Mol Cell Cardiol 137:119-131 (2019). PubMed: 31669609
  • Maruyama K  et al. Age-related changes to vascular protease-activated receptor 2 in metabolic syndrome: a relationship between oxidative stress, receptor expression, and endothelium-dependent vasodilation. Can J Physiol Pharmacol 95:356-364 (2017). PubMed: 28103056
  • Tsihlis ND  et al. Nitric oxide is less effective at inhibiting neointimal hyperplasia in spontaneously hypertensive rats. Nitric Oxide 35:165-74 (2013). IHC-P ; Rat . PubMed: 24149190
  • van Heerebeek L  et al. Low myocardial protein kinase g activity in heart failure with preserved ejection fraction. Circulation 126:830-9 (2012). IHC-P ; Human . PubMed: 22806632
  • Succu S  et al. Oxytocin induces penile erection when injected into the ventral tegmental area of male rats: role of nitric oxide and cyclic GMP. Eur J Neurosci 28:813-21 (2008). IHC-FoFr ; Rat . PubMed: 18671741

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Guanylyl Cyclase beta 1 antibody

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Right Heart Atria)
Loading amount
20 µg
Specification
Right Heart Atria
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 22 2012

Question

ab24824 inWB zu testen

Read More
Answer

DISCOUNT CODE: 100%ABR-0000
Ablaufdatum: 27. Januar 2013

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab24824 inWB zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer 100%ABR-0000, deckt den Wert von ab24824 ab und kann zum Kauf weiterer Produkte eingesetzt werden.

Der Code wird gültig, sobald Sie uns ein Abreview mitAbbildungfür diesen Antikörper über den Test in WB eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com