Anti-GUCY1A2 抗体 (ab42108)
Key features and details
- Rabbit polyclonal to GUCY1A2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-GUCY1A2 antibody -
製品の詳細
Rabbit polyclonal to GUCY1A2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human GUCY1A2 aa 692-708.
Sequence:GICYFLEVR TGPKPPKP
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Antibody stabilization buffer, with preservatives, consisting of Hepes, NaCl, KCl, glycerol, BSA and chaotropic agents. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab42108の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/150.
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| WB |
1/750. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
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| 特記事項 |
|---|
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IHC-P
1/50 - 1/150. |
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WB
1/750. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa). |
ターゲット情報
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機能
Has guanylyl cyclase on binding to the beta-1 subunit.
Isoform 2 acts as a negative regulator of guanylyl cyclase activity as it forms non-functional heterodimers with the beta subunits. -
組織特異性
Isoform 1 is expressed in fetal brain, liver, colon, endothelium and testis. Isoform 2 is expressed only in liver, colon and endothelium. -
配列類似性
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 1 guanylate cyclase domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 234889 Mouse
- Entrez Gene: 66012 Rat
- SwissProt: Q9WVI4 Rat
- Unigene: 209304 Rat
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別名
- GC SA2 antibody
- GCS alpha 2 antibody
- GCS-alpha-2 antibody
see all
画像
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Western blot - Anti-GUCY1A2 antibody (ab42108)Anti-GUCY1A2 antibody (ab42108) at 1/750 dilution + Soluble Guanylyl Cyclase alpha 2 Antibody Positive Control (Affinity Purified Recombinant full length protein)
Predicted band size: 98 kDa
Observed band size: 98 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1A2 antibody (ab42108)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse large intestine staining GUCY1A2 with ab42108 at a 1/100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnificiation.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab42108 は 8 報の論文で使用されています。
- Gartside SE et al. A role for nitric oxide in serotonin neurons of the midbrain raphe nuclei. Eur J Neurosci 51:1881-1899 (2020). PubMed: 32115781
- Zhan R et al. Nitric oxide induces epidermal stem cell de-adhesion by targeting integrin ß1 and Talin via the cGMP signalling pathway. Nitric Oxide 78:1-10 (2018). WB . PubMed: 29698689
- Datunashvili M et al. Modulation of Hyperpolarization-Activated Inward Current and Thalamic Activity Modes by Different Cyclic Nucleotides. Front Cell Neurosci 12:369 (2018). PubMed: 30405353
- Olthof BM et al. Puncta of neuronal nitric oxide synthase (nNOS) mediate NMDA-receptor signalling in the auditory midbrain. J Neurosci N/A:N/A (2018). PubMed: 30530507
- Thoonen R et al. Cardiovascular and pharmacological implications of haem-deficient NO-unresponsive soluble guanylate cyclase knock-in mice. Nat Commun 6:8482 (2015). PubMed: 26442659
- Buys ES et al. sGC(alpha)1(beta)1 attenuates cardiac dysfunction and mortality in murine inflammatory shock models. Am J Physiol Heart Circ Physiol 297:H654-63 (2009). WB ; Mouse . PubMed: 19502556
- De Backer O et al. Role of the soluble guanylyl cyclase alpha1/alpha2 subunits in the relaxant effect of CO and CORM-2 in murine gastric fundus. Naunyn Schmiedebergs Arch Pharmacol 378:493-502 (2008). WB ; Mouse . PubMed: 18563392
- Chicoine LG et al. Maturational changes in the regulation of pulmonary vascular tone by nitric oxide in neonatal rats. Am J Physiol Lung Cell Mol Physiol 293:L1261-70 (2007). PubMed: 17827249
