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AB32417

Anti-GTPase HRAS 抗体 [Y132]

Anti-GTPase HRAS antibody [Y132]

3

(6 Reviews)

|

(18 Publications)

Anti-GTPase HRAS antibody [Y132] (ab32417) is a rabbit monoclonal antibody detecting GTPase HRAS in Western Blot, IP. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
- Trusted since 2006

別名を表示する

HRAS1, HRAS, GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras

5 Images
Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417)
  • WB

Unknown

Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417)

All lanes:

Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/500 dilution

Lane 1:

MCF7 cell lysate

Lane 2:

PC12 cell lysate

Predicted band size: 21 kDa

Observed band size: 21 kDa

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Immunoprecipitation - Anti-GTPase HRAS antibody [Y132] (AB32417)
  • IP

Unknown

Immunoprecipitation - Anti-GTPase HRAS antibody [Y132] (AB32417)

ab32417 (purified) at 1/60 immunoprecipitating GTPase in 10 μg mouse brain whole cell lysate (Lanes 1 and 2, observed at 21 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP Detection Reagent (ab131366) was used for detection (1/10 000). Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-GTPase HRAS antibody [Y132] (ab32417)

Predicted band size: 21 kDa

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Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417)
  • WB

Lab

Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417)

Lanes 1 - 4 : Merged signal (red and green). Green - ab32417 observed at 21 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab32417 was shown to recognize HRAS in wild-type HEK-293 cells as signal was lost at the expected MW in HRAS knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and HRAS knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab32417 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/500 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

HRAS knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human HRAS knockout HEK-293 cell line (<a href='/products/cell-lines/human-hras-knockout-hek-293-cell-line-ab260858'>ab260858</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 21 kDa

false

Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417)
  • WB

Lab

Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417)
  • WB

Lab

Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/2500 dilution

Lane 1:

mouse brain lysate at 10 µg

Lane 2:

rat brain lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-GTPase HRAS antibody [Y132] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

Y132

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Reactivity with other RAS members has not been tested.

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Reactivity data

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下記製品にもご興味をお持ちいただけるかもしれません:

AB267797

Human HRAS ELISA Kit

0

0 Reviews

製品ページを見る
こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

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製品の詳細

What is this antibody validated in?
Anti-GTPase HRAS antibody [Y132] (ab32417) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-GTPase HRAS [Y132] (ab32417) was first used in a scientific publication in 2006 and has been cited over 10 times in peer-reviewed journals.

Reviewed by scientists
Anti-GTPase HRAS [Y132] (ab32417) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-GTPase HRAS antibody [Y132] (ab32417) has been confirmed by Western blot testing in HRAS Knockout HEK-293 cells.

Other related products
We have a range of other formats of antibody clone [Y132] also available for your convenience: ab32417, Carrier free - ab239815

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GTPase HRAS also known as H-RAS or HRAS1 is a small GTPase enzyme involved in intracellular signal transduction. It has a molecular mass of approximately 21 kDa. HRAS is widely expressed in various tissues and plays a role in regulating cell growth and differentiation. The HRAS protein functions as a molecular switch cycling between an active GTP-bound state and an inactive GDP-bound state. This activity is facilitated by the HRAS protein's intrinsic GTPase activity which hydrolyzes GTP to GDP.
Biological function summary

GTPase HRAS participates in the regulation of cell proliferation differentiation and survival serving as an essential component of several signal transduction complexes. HRAS interacts with various signaling molecules and pathways to mediate mitogenic signals. Through these interactions the HRAS protein modulates cellular responses to extracellular stimuli influencing processes like growth and division playing an important role in maintaining normal cellular functions.

Pathways

The involvement of GTPase HRAS in the MAPK/ERK and the PI3K/AKT pathways illustrates its importance in transmitting signals from cell surface receptors. These pathways control a variety of cellular functions including growth survival and metabolism. HRAS interacts with related proteins like KRAS and NRAS integral members of the RAS family. These interactions highlight its role in signaling cascades that contribute to the cellular response to growth factors.

Mutations and aberrant activation of GTPase HRAS link to oncogenic processes particularly in bladder and thyroid cancers. The mutated HRAS protein contributes to the unchecked cellular proliferation seen in tumorigenesis. Additionally HRAS mutations associate with developmental disorders such as Costello syndrome where aberrant signaling disrupts normal developmental processes. HRAS-related pathways often involve other RAS family members like KRAS underlining their collective impact in disease states.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in the activation of Ras protein signal transduction (PubMed : 22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed : 12740440, PubMed : 14500341, PubMed : 9020151).
See full target information HRAS
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文献 (18)

Recent publications for all applications. Explore the full list and refine your search

BMC ophthalmology 22:456 PubMed36443719

2022

Blue light attenuates TGF-β2-induced epithelial-mesenchymal transition in human lens epithelial cells via autophagy impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyan Zhang,Hong Zhu,Xin Yu,Liyin Wang,Yingying Wen,Liyue Zhang,Jianping Tong,Ye Shen

NPJ precision oncology 6:86 PubMed36418474

2022

Co-targeting KRAS G12C and EGFR reduces both mutant and wild-type RAS-GTP.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas McFall,Michael Trogdon,Anita C Guizar,John F Langenheim,Laura Sisk-Hackworth,Edward C Stites

Cell reports 37:110096 PubMed34910921

2021

Identification of RAS mutant biomarkers for EGFR inhibitor sensitivity using a systems biochemical approach.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas McFall,Edward C Stites

Nature communications 11:5312 PubMed33082319

2020

Siah2 integrates mitogenic and extracellular matrix signals linking neuronal progenitor ciliogenesis with germinal zone occupancy.

Applications

Unspecified application

Species

Unspecified reactive species

Taren Ong,Niraj Trivedi,Randall Wakefield,Sharon Frase,David J Solecki

Molecular and cellular biochemistry 476:175-186 PubMed32970285

2020

CircZNF609 promotes cell proliferation, migration, invasion, and glycolysis in nasopharyngeal carcinoma through regulating HRAS via miR-338-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Zhonglu Liu,Feifei Liu,Fang Wang,Xin Yang,Wentao Guo

Evidence-based complementary and alternative medic 2020:2760979 PubMed32419796

2020

Ethanol Extract of the Infructescence of Sieb. et Zucc. Induces Methuosis of Human Nasopharyngeal Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jinkun Liu,Min Ying,Bin Wu,Chaomei Fu

Theranostics 9:6175-6190 PubMed31534544

2019

DNA methylation-regulated QPCT promotes sunitinib resistance by increasing HRAS stability in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tangliang Zhao,Yi Bao,Xinxin Gan,Jie Wang,Qiong Chen,Zhihui Dai,Bing Liu,Anbang Wang,Shuhan Sun,Fu Yang,Linhui Wang

Cell death & disease 7:e2311 PubMed27468687

2016

PGA1-induced apoptosis involves specific activation of H-Ras and N-Ras in cellular endomembranes.

Applications

WB

Species

Unspecified reactive species

B Anta,A Pérez-Rodríguez,J Castro,C A García-Domínguez,S Ibiza,N Martínez,L M Durá,S Hernández,T Gragera,D Peña-Jiménez,M Yunta,N Zarich,P Crespo,J M Serrador,E Santos,A Muñoz,J L Oliva,J M Rojas-Cabañeros

The Journal of neuroscience : the official journal of the Society for Neuroscience 36:6431-44 PubMed27307232

2016

Identification of PSD-95 Depalmitoylating Enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Norihiko Yokoi,Yuko Fukata,Atsushi Sekiya,Tatsuro Murakami,Kenta Kobayashi,Masaki Fukata

Leukemia 30:1542-51 PubMed27055865

2016

Loss of wild-type Kras promotes activation of all Ras isoforms in oncogenic Kras-induced leukemogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

G Kong,Y-I Chang,A Damnernsawad,X You,J Du,E A Ranheim,W Lee,M-J Ryu,Y Zhou,Y Xing,Q Chang,C E Burd,J Zhang
View all publications
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