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リコンビナントRabMAb

Anti-GTF2E2 抗体 [EPR14929] (ab187143)

Submit a review Submit a question References (4)
Western blot - Anti-GTF2E2 antibody [EPR14929] (ab187143)
  • Western blot - Anti-GTF2E2 antibody [EPR14929] (ab187143)
  • Immunoprecipitation - Anti-GTF2E2 antibody [EPR14929] (ab187143)
  • Immunocytochemistry/ Immunofluorescence - Anti-GTF2E2 antibody [EPR14929] (ab187143)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2E2 antibody [EPR14929] (ab187143)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2E2 antibody [EPR14929] (ab187143)
  • Anti-GTF2E2 antibody [EPR14929] (ab187143)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14929] to GTF2E2
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-GTF2E2 antibody [EPR14929]
    GTF2E2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14929] to GTF2E2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • K562, Jurkat, 293T, mouse heart, rat heart and rat brain lysates; Human cervix carcinoma and mouse liver tissues; 293T cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187143の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
IP
1/30 - 1/50.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
IP
1/30 - 1/50.

ターゲット情報

  • 機能

    Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

  • 配列類似性

    Belongs to the TFIIE beta subunit family.
    Contains 1 TFIIE beta DNA-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FE antibody
      • General transcription factor IIE subunit 2 antibody
      • General transcription factor IIE, polypeptide 2, beta 34kDa antibody
      • gtf2e2 antibody
      • T2EB_HUMAN antibody
      • TF2E 2 antibody
      • TF2E2 antibody
      • TFIIE B antibody
      • TFIIE-beta antibody
      • Transcription initiation factor IIE beta subunit antibody
      • Transcription initiation factor IIE subunit beta antibody
      see all

    画像

    • Western blot - Anti-GTF2E2 antibody [EPR14929] (ab187143)
      Western blot - Anti-GTF2E2 antibody [EPR14929] (ab187143)
      All lanes : Anti-GTF2E2 antibody [EPR14929] (ab187143) at 1/10000 dilution

      Lane 1 : K562 lysate
      Lane 2 : Jurkat lysate
      Lane 3 : 293T lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 33 kDa
      Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-GTF2E2 antibody [EPR14929] (ab187143)
      Western blot - Anti-GTF2E2 antibody [EPR14929] (ab187143)
      All lanes : Anti-GTF2E2 antibody [EPR14929] (ab187143) at 1/1000 dilution

      Lanes 1 & 3 : Mouse heart tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 33 kDa
      Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands.

    • Immunoprecipitation - Anti-GTF2E2 antibody [EPR14929] (ab187143)
      Immunoprecipitation - Anti-GTF2E2 antibody [EPR14929] (ab187143)

      Immunoprecipitation. ab187143 at 1/50 labeling GTF2E2 immunoprecipitated from Jurkat cell lysate using ab187143 at 1/30.

    • Immunocytochemistry/ Immunofluorescence - Anti-GTF2E2 antibody [EPR14929] (ab187143)
      Immunocytochemistry/ Immunofluorescence - Anti-GTF2E2 antibody [EPR14929] (ab187143)

      Immunofluorescent analysis of 293T cells labeling GTF2E2 with ab187143 at 1/250 and DAPI staining (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2E2 antibody [EPR14929] (ab187143)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2E2 antibody [EPR14929] (ab187143)

      Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling GTF2E2 with ab187143 at 1/250 with a prediluted HRP Polymer for Rabbit IgG as a secondary antibody.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2E2 antibody [EPR14929] (ab187143)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2E2 antibody [EPR14929] (ab187143)

      Immunohistochemical analysis of paraffin embedded Human cervix carcinoma tissue labeling GTF2E2 with ab187143 at 1/250 with prediluted HRP Polymer for Rabbit IgG as a secondary antibody.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-GTF2E2 antibody [EPR14929] (ab187143)
      Anti-GTF2E2 antibody [EPR14929] (ab187143)

    参考文献 (4)

    ab187143 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab187143 は 4 報の論文で使用されています。

    • Zhang Y  et al. GTF2E2 is a novel biomarker for recurrence after surgery and promotes progression of esophageal squamous cell carcinoma via miR-139-5p/GTF2E2/FUS axis. Oncogene 41:782-796 (2022). PubMed: 34853466
    • Ishida R  et al. Mayaro Virus Non-Structural Protein 2 Circumvents the Induction of Interferon in Part by Depleting Host Transcription Initiation Factor IIE Subunit 2. Cells 10:N/A (2021). PubMed: 34944018
    • Gonçalves E  et al. Widespread Post-transcriptional Attenuation of Genomic Copy-Number Variation in Cancer. Cell Syst 5:386-398.e4 (2017). PubMed: 29032074
    • Theil AF  et al. Trichothiodystrophy causative TFIIEß mutation affects transcription in highly differentiated tissue. Hum Mol Genet 26:4689-4698 (2017). PubMed: 28973399

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