Anti-GSTZ1 抗体 (ab153995)
Key features and details
- Rabbit polyclonal to GSTZ1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GSTZ1 antibody -
製品の詳細
Rabbit polyclonal to GSTZ1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Pig
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免疫原
Recombinant fragment corresponding to Human GSTZ1 aa 1-250.
Database link: O43708 -
ポジティブ･コントロール
- 293T; A431; H1299; HeLa; HepG2; MOLT4 and Raji cell lysates. SW480 xenograft; A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153995の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 24 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate Buffer
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 24 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate Buffer |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid. -
組織特異性
Mostly expressed in liver followed by kidney, skeletal muscle and brain. Also expressed in melanocytes, synovium, placenta, breast and fetal liver and heart. -
パスウェイ
Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 5/6. -
配列類似性
Belongs to the GST superfamily. Zeta family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 2954 Human
- Entrez Gene: 100626791 Pig
- Omim: 603758 Human
- SwissProt: O43708 Human
- Unigene: 655292 Human
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別名
- EC 2.5.1.18 antibody
- EC 5.2.1.2 antibody
- Glutathione S transferase zeta 1 antibody
see all
画像
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Western blot - Anti-GSTZ1 antibody (ab153995)Anti-GSTZ1 antibody (ab153995) at 1/1000 dilution + 293T cell lysate at 30 µg
Predicted band size: 24 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTZ1 antibody (ab153995)Immunohistochemical analysis of paraffin- embedded SW480 xenograft labeling GSTZ1 using ab153995 at 1/100 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-GSTZ1 antibody (ab153995)Immunofluorescence analysis of methanol-fixed A431 cells, labeling GSTZ1 using ab153995 at 1/200 dilution. Right panel costained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab153995 は 2 報の論文で使用されています。
- Shen J et al. Identification of a Novel Target Implicated in Chronic Obstructive Sleep Apnea-Related Atrial Fibrillation by Integrative Analysis of Transcriptome and Proteome. J Inflamm Res 16:5677-5695 (2023). PubMed: 38050561
- Alaidaroos NYA et al. Differential SOD2 and GSTZ1 profiles contribute to contrasting dental pulp stem cell susceptibilities to oxidative damage and premature senescence. Stem Cell Res Ther 12:142 (2021). PubMed: 33596998