Anti-GSTM1/2/4/5 抗体 [EPR13338] (ab178684)
![Western blot - Anti-GSTM1/2/4/5 antibody [EPR13338] (ab178684)](https://www.abcam.co.jp/ps/products/178/ab178684/Images/ab178684-206900-anti-gstm1-2-4-5-antibody-epr13338-western-blot.jpg "Western blot - Anti-GSTM1/2/4/5 antibody [EPR13338] (ab178684)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13338] to GSTM1/2/4/5
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GSTM1/2/4/5 antibody [EPR13338]
GSTM1/2/4/5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13338] to GSTM1/2/4/5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human fetal brain, Human testis, HeLa and HepG2 lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR13338 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab178684の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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| IP |
1/10 - 1/100.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
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IP
1/10 - 1/100. |
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
画像
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Western blot - Anti-GSTM1/2/4/5 antibody [EPR13338] (ab178684)All lanes : Anti-GSTM1/2/4/5 antibody [EPR13338] (ab178684) at 1/1000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human testis lysates
Lane 3 : HeLa lysates
Lane 4 : HepG2 lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
![Immunoprecipitation - Anti-GSTM1/2/4/5 antibody [EPR13338] (ab178684)](https://www.abcam.co.jp/ps/products/178/ab178684/Images/ab178684-206899-anti-gstm1-2-4-5-antibody-epr13338-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GSTM1/2/4/5 antibody [EPR13338] (ab178684)Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal brain lysate or (Lane 2) 1X PBS (negative control) immunoprecipitated using ab178684 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Anti-GSTM1/2/4/5 antibody [EPR13338] (ab178684)](https://www.abcam.co.jp/ps/products/178/ab178684/Images/ab178684-3-benefits-of-recombinant-antibodies.png)
Anti-GSTM1/2/4/5 antibody [EPR13338] (ab178684)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab178684 は 5 報の論文で使用されています。
- Qiao Z et al. Dysfunction of ATG7-dependent autophagy dysregulates the antioxidant response and contributes to oxidative stress-induced biological impairments in human epidermal melanocytes. Cell Death Discov 6:31 (2020). PubMed: 32377394
- Smirlis D et al. SILAC-based quantitative proteomics reveals pleiotropic, phenotypic modulation in primary murine macrophages infected with the protozoan pathogen Leishmania donovani. J Proteomics 213:103617 (2020). PubMed: 31846769
- Dittmann A et al. High-fat diet in a mouse insulin-resistant model induces widespread rewiring of the phosphotyrosine signaling network. Mol Syst Biol 15:e8849 (2019). PubMed: 31464373
- Shanmugam G et al. Constitutive activation of Nrf2 induces a stable reductive state in the mouse myocardium. Redox Biol 12:937-945 (2017). WB ; Mouse . PubMed: 28482326
- Kumar RR et al. Abrogation of Nrf2 impairs antioxidant signaling and promotes atrial hypertrophy in response to high-intensity exercise stress. J Transl Med 14:86 (2016). IHC-Fr ; Mouse . PubMed: 27048381