AB153949

Anti-GST3 / GST pi 抗体

Name: Anti-GST3 / GST pi 抗体 (ab153949) | Abcam
Brand: Abcam Limited
SKU: AB153949
Price: 78000 JPY
Availability: InStock
Rating: 4 (4 reviews)

Anti-GST3 / GST pi antibody

(4 Reviews)

(11 Publications)

Rabbit Polyclonal GST3 / GST pi antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GSTP1 aa 1-200.

別名を表示する

FAEES3, GST3, GSTP1, Glutathione S-transferase P, GST class-pi, GSTP1-1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB153949)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB153949)

Immunohistochemical analysis of paraffin-embedded U87 xenograft, labeling GST3 / GST pi with ab153949 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody (AB153949)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody (AB153949)

Immunofluorescent analysis methanol fixed HeLa cells; labeling GST3 / GST pi with ab153949 at 1/200 dilution. Blue : Hoechst 33343 staining

Unknown

Western blot - Anti-GST3 / GST pi antibody (AB153949)

12% SDS PAGE

All lanes:

Western blot - Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

Molt-4 whole cell lysate at 30 µg

Predicted band size: 23 kDa

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Unknown

Western blot - Anti-GST3 / GST pi antibody (AB153949)

12 % SDS-PAGE

All lanes:

Western blot - Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution

All lanes:

Rat brain whole cell lysate at 50 µg

Predicted band size: 23 kDa

false

Unknown

Western blot - Anti-GST3 / GST pi antibody (AB153949)

12% SDS PAGE

All lanes:

Western blot - Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 23 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IHC-P, WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human GSTP1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P09211

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GST3 also known as GST pi or GSTP is a protein part of the glutathione S-transferase family. These enzymes are involved in detoxifying processes by catalyzing the conjugation of glutathione to harmful substances. GSTP is approximately 23 kDa in size and is predominantly expressed in mammalian tissues including the liver and lung. These tissues play active roles in detoxifying external chemicals and managing oxidative stress in the body.

Biological function summary

GST3 contributes to the detoxification of xenobiotics and electrophilic compounds playing a protective role against cellular damage. It can exist as homo-dimers or form hetero-dimers with other GST family members participating in larger enzyme complexes. Through conjugation reactions GSTP helps in the detoxification processes thereby aiding cellular defenses and maintaining cellular redox balance.

Pathways

GST3 is an integral part of the phase II detoxification pathway and oxidative stress response. It interacts closely with other proteins such as Nrf2 (Nuclear factor erythroid 2-related factor 2) and helps regulate the transcription of antioxidant proteins that protect against oxidative damage. This enzyme plays a role in modulating the MAPK signaling pathway which influences cell proliferation and apoptosis.

GSTP has links to several cancer types including liver and lung cancer. Its overexpression in tumors suggests its role in cancer cell survival and resistance to chemotherapy. GSTP interacts with cancer-associated proteins like c-Jun N-terminal kinase (JNK) influencing pathways that lead to aberrant cellular growth and transformation. It also has associations with neurodegenerative diseases given its role in counteracting oxidative stress which commonly underpins neurodegeneration.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.

See full target information GSTP1

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1559303 PubMed40786041

2025

Dipeptidyl peptidase-4 enzyme inhibition and its impacts on hepatic preneoplasia: a new avenue for liver cancer management.

Applications

Unspecified application

Species

Unspecified reactive species

Hebatollah E Eitah,Rabab H Sayed,Yousreya A Maklad,Amina A Gamal El Din,Khaled Mahmoud,Ayman E El-Sahar,Amani Alhejely,Amal A Abdulbaqi,Hanan Naeim Attia

Cell death & disease 14:463 PubMed37491277

2023

A GSTP1-mediated lactic acid signaling promotes tumorigenesis through the PPP oxidative branch.

Applications

Unspecified application

Species

Unspecified reactive species

Yandi Sun,Qian He,Jingjia Li,Ze Yang,Mashaal Ahmad,Yindan Lin,Di Wu,Lei Zheng,Jiangtao Li,Ben Wang,Chitty Chen,Yue Hu,Heng Luo,Yan Luo

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:8644-8667 PubMed34493542

2021

Critical Role of Astrocyte NAD Glycohydrolase in Myelin Injury and Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Monica R Langley,Chan-Il Choi,Thais R Peclat,Yong Guo,Whitney L Simon,Hyesook Yoon,Laurel Kleppe,Claudia F Lucchinetti,Claudia C S Chini,Eduardo N Chini,Isobel A Scarisbrick

Open life sciences 16:667-681 PubMed34250246

2021

Long noncoding RNA HULC contributes to paclitaxel resistance in ovarian cancer via miR-137/ITGB8 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Huang,Min Wei,Li Hong

Glia 69:2111-2132 PubMed33887067

2021

The thrombin receptor modulates astroglia-neuron trophic coupling and neural repair after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ha Neui Kim,Erin M Triplet,Maja Radulovic,Samantha Bouchal,Laurel S Kleppe,Whitney L Simon,Hyesook Yoon,Isobel A Scarisbrick

Oncology letters 20:23 PubMed32774496

2020

MicroRNA-212-3p inhibits paclitaxel resistance through regulating epithelial-mesenchymal transition, migration and invasion by targeting ZEB2 in human hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jianyu Yang,Ronghua Cui,Yingke Liu

Journal of cellular physiology 235:8472-8485 PubMed32329523

2020

Long intergenic noncoding RNA 00844 promotes apoptosis and represses proliferation of prostate cancer cells through upregulating GSTP1 by recruiting EBF1.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyan Qiu,Zhixiong Zheng,Yingfu Huang

Therapeutic advances in medical oncology 11:1758835919838958 PubMed31019568

2019

Overexpression of long noncoding RNA LINC01419 in esophageal squamous cell carcinoma and its relation to the sensitivity to 5-fluorouracil by mediating GSTP1 methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Liang Chen,Zhi-Xiong Lin,Yun-Sheng Qin,Yu-Qi She,Yun Chen,Chen Chen,Guo-Dong Qiu,Jie-Ting Zheng,Zhong-Lin Chen,Shu-Yao Zhang

Molecular genetics & genomic medicine 6:819-827 PubMed30043549

2018

Evaluation of glutathione S-transferase pi 1 expression and gene promoter methylation in Moroccan patients with urothelial bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Khaoula Hadami,Nadia Dakka,Mounia Bensaid,Hajar El Ahanidi,Ahmed Ameur,Hafsa Chahdi,Mohamed Oukabli,Abderrahmane Al Bouzidi,Mohammed Attaleb,Mohammed El Mzibri

Cancer biology & therapy 18:177-185 PubMed28278080

2017

Hypoxia promotes chemotherapy resistance by down-regulating SKA1 gene expression in human osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Ma,Yinglong Zhang,Tao Liu,Kuo Jiang,Yanhua Wen,Qingyu Fan,Xiuchun Qiu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GST3 / GST pi 抗体

Anti-GST3 / GST pi antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB153949)

Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody (AB153949)

Western blot - Anti-GST3 / GST pi antibody (AB153949)

Western blot - Anti-GST3 / GST pi antibody (AB153949)

Western blot - Anti-GST3 / GST pi antibody (AB153949)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (11)

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