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リコンビナントRabMAb

Anti-GST 抗体 [EPR4236] (ab111947)

Submit a review Submit a question References (23)
Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (ab111947)
  • Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
  • Western blot - Anti-GST antibody [EPR4236] (ab111947)
  • Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
  • Western blot - Anti-GST antibody [EPR4236] (ab111947)
  • Anti-GST antibody [EPR4236] (ab111947)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4236] to GST
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Species independent

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-GST antibody [EPR4236]
    GST 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4236] to GST

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, IPmore details
    適用なし: Flow Cyt or IHC-P

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • GST fusion proteins; Alpha-actinin-4-GST, AKT1/PKBa-GST or CDK7-GST.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab111947の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
ICC/IF
1/100.
IP
1/20.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
ICC/IF
1/100.
IP
1/20.
追加情報
Is unsuitable for Flow Cyt or IHC-P.

ターゲット情報

  • 関連性

    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

  • 別名

    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (ab111947)
    Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (ab111947)

    Immunofluorescence staining of 293T cells transfected with GST, with ab111947 at a working dilution of 1/100 and counter-stained with DAPI. Tubulin was also stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1/500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X-100. The negative controls are shown in the bottom hand panels - for the first negative control, anti-GST antibody (ab111947) was used at a dilution of 1/100 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/1000 and for the second negative control, mouse anti-tubulin (ab7291) and anti-rabbit secondary antibody (ab150077) were used.

  • Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
    Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)

    GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

    Lane 1: Primary: ab111947, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
    Lane 2: Primary: ab111947, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate
    Lane 3: Primary: ab172730, Rabbit monoclonal IgG
    Sample: 293T transfected with GST tagged protein vector cell lysate

    All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
    Blocking and diluting buffer: 5% NFDM/TBST



    Lanes 1-2 : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
    Lane 3 : Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Lane 1 : Input - 293 transfected with GST tagged protein vector cell lysate at 10 µg
    Lane 2 : (+) 293T transfected with GST tagged protein vector cell lysate
    Lane 3 : (-) 293T transfected with GST tagged protein vector cell lysate

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution (VeriBlot)

    Additional bands at: 41 kDa (possible tagged protein)

  • Western blot - Anti-GST antibody [EPR4236] (ab111947)
    Western blot - Anti-GST antibody [EPR4236] (ab111947)
    Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution + GST recombinant protein at 0.01 µg

    Predicted band size: 26 kDa

  • Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
    Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)

    (Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

    Lane 1: Primary: ab180123, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
    Lane 2: Primary: ab180123, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate
    Secondary: ab131366, Veriblot, 1/10000 dilution
    Lane 3: Primary: ab172730, Rabbit monoclonal IgG
    Sample: 293T transfected with GST tagged protein vector cell lysate

    All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-GST antibody [EPR4236] (ab111947)
    Western blot - Anti-GST antibody [EPR4236] (ab111947)
    All lanes : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution

    Lane 1 : Alpha-actinin-4-GST fusion protein
    Lane 2 : AKT1/PKBa-GST fusion protein
    Lane 3 : CDK7-GST fusion protein

    Lysates/proteins at 0.01 µg per lane.

    Predicted band size: 26 kDa

  • Anti-GST antibody [EPR4236] (ab111947)
    Anti-GST antibody [EPR4236] (ab111947)

参考文献 (23)

ab111947 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab111947 は 23 報の論文で使用されています。

  • Jeon HS  et al. Pathogen-induced autophagy regulates monolignol transport and lignin formation in plant immunity. Autophagy 19:597-615 (2023). PubMed: 35652914
  • Wu Y  et al. Metabolite asymmetric dimethylarginine (ADMA) functions as a destabilization enhancer of SOX9 mediated by DDAH1 in osteoarthritis. Sci Adv 9:eade5584 (2023). PubMed: 36753544
  • Wang Q  et al. ERα promotes SUMO1 transcription by binding with the ERE and enhances SUMO1-mediated protein SUMOylation in breast cancer. Gland Surg 12:963-973 (2023). PubMed: 37727335
  • Berk JM  et al. OtDUB from the Human Pathogen Orientia tsutsugamushi Modulates Host Membrane Trafficking by Multiple Mechanisms. Mol Cell Biol 42:e0007122 (2022). PubMed: 35727026
  • Yang G  et al. The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling. Front Microbiol 13:911479 (2022). PubMed: 35770165
View all Publications for this product

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