Anti-GST 抗体 (ab181652)
Key features and details
- Goat polyclonal to GST
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-GST antibody
GST 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to GST -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Species independent -
免疫原
Full length protein corresponding to Schistosoma japonicum GST aa 1 to the C-terminus. Native Protein
Database link: P08515 -
ポジティブ･コントロール
- Recombinant Human GST protein (ab81793) can be used as a positive control in WB. Glutathione-S-Transferase [Schistosoma japonicum]
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
IgG fraction -
特記事項（精製）
ab181652 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181652の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. |
ターゲット情報
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関連性
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
別名
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
画像
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Western blot - Anti-GST antibody (ab181652)Lanes 1-4 : Anti-GST antibody (ab181652) at 1/5000 dilution
Lane 5 : Anti-GST antibody (ab181652) at 1/1000 dilution (Reduced)
Lane 6 : Anti-GST antibody (ab181652) at 1/1000 dilution (Non-reduced)
Lanes 1 & 3 & 5-6 : Purified GST at 1 µg
Lanes 2 & 4 : Purified GST at 0.25 µgab181652 was used to detect GST under reducing and non-reducing conditions. Reduced samples of purified GST contained 4% BME and were boiled for 5 minutes. For lanes 1-4, samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with a Goat anti GST at 1/5000 dilution overnight at 4°C. Primary antibody was detected with a conjugated Donkey anti Goat at 1/10000, incubated for 1.5 hr at RT and imaged on the BioRad VersaDoc imaging system. Lanes 5-6 show a repeat western blot with the same samples (~1 ug per lane, reduced (R) and nonreduced (NR) detected with a Dylight 549 conjugated Donkey anti goat incubated at 1/10000 at 1.5 hours at RT.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab181652 は 1 報の論文で使用されています。
- Marks CR et al. Activated CaMKIIa Binds to the mGlu5 Metabotropic Glutamate Receptor and Modulates Calcium Mobilization. Mol Pharmacol 94:1352-1362 (2018). PubMed: 30282777