Key features and details
- Goat polyclonal to GST
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
GST 一次抗体 製品一覧
製品の詳細Goat polyclonal to GST
アプリケーション適用あり: WBmore details
種交差性交差種: Species independent
- Recombinant Human GST protein (ab81793) can be used as a positive control in WB. Glutathione-S-Transferase [Schistosoma japonicum]
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファーPreservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
特記事項（精製）ab181652 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
The Abpromise guarantee
1/1000 - 1/5000.
1/1000 - 1/5000.
関連性GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
Lanes 1-4 : Anti-GST antibody (ab181652) at 1/5000 dilution
Lane 5 : Anti-GST antibody (ab181652) at 1/1000 dilution (Reduced)
Lane 6 : Anti-GST antibody (ab181652) at 1/1000 dilution (Non-reduced)
Lanes 1 & 3 & 5-6 : Purified GST at 1 µg
Lanes 2 & 4 : Purified GST at 0.25 µg
ab181652 was used to detect GST under reducing and non-reducing conditions. Reduced samples of purified GST contained 4% BME and were boiled for 5 minutes. For lanes 1-4, samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with a Goat anti GST at 1/5000 dilution overnight at 4°C. Primary antibody was detected with a conjugated Donkey anti Goat at 1/10000, incubated for 1.5 hr at RT and imaged on the BioRad VersaDoc imaging system. Lanes 5-6 show a repeat western blot with the same samples (~1 ug per lane, reduced (R) and nonreduced (NR) detected with a Dylight 549 conjugated Donkey anti goat incubated at 1/10000 at 1.5 hours at RT.
ab181652 は 1 報の論文で使用されています。
- Marks CR et al. Activated CaMKIIa Binds to the mGlu5 Metabotropic Glutamate Receptor and Modulates Calcium Mobilization. Mol Pharmacol 94:1352-1362 (2018). PubMed: 30282777