Anti-GST 抗体 [3D4] (ab34589)
Key features and details
- Mouse monoclonal [3D4] to GST
- Suitable for: ELISA, WB
- Reacts with: Species independent
- Isotype: IgG2b
製品の概要
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製品名
Anti-GST antibody [3D4]
GST 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3D4] to GST -
由来種
Mouse -
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Species independent -
免疫原
Glutathione S Transferase (Schistosoma japonicum).
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ポジティブ・コントロール
- WB: HeLa whole cell lysate (ab150035).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Anti-GST monoclonal antibody was purified from concentrated tissue culture supernate by Protein A chromatography which reacts specifically with GST. -
ポリ/モノ
モノクローナル -
クローン名
3D4 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34589の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
1/50000 - 1/150000.
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|
WB |
1/1000 - 1/10000. Detects a band of approximately 26 kDa.
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特記事項 |
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ELISA
1/50000 - 1/150000. |
WB
1/1000 - 1/10000. Detects a band of approximately 26 kDa. |
ターゲット情報
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関連性
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
別名
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
画像
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Western blot using mouse monoclonal [3D4] anti-GST antibody (ab34589) to detect purified GST recombinant protein (left) and in a lysate of HeLa cells expressing recombinant GST protein (right). Approximately 0.1µg of protein was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with primary antibody diluted to 1.0µg/ml for 1 h at room temperature, followed by washes and reaction with a 1:5000 dilution of IRDye 800 conjugated Gt-a-Mouse IgG [H&L] MX. The fluorescence image was captured using a LI-COR imaging system. Band size: approximately 26kDa.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab34589 は 2 報の論文で使用されています。
- Sun Y et al. Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic Escherichia coli and Contributes to Opsonophagocytosis Resistance in Bacteria. Front Cell Infect Microbiol 10:592906 (2020). PubMed: 33569353
- Nakashima H et al. Interferon-stimulated gene 15 (ISG15) and ISG15-linked proteins can associate with members of the selective autophagic process, histone deacetylase 6 (HDAC6) and SQSTM1/p62. J Biol Chem 290:1485-95 (2015). PubMed: 25429107