Anti-GSK3 beta (phospho S9) 抗体 (ab107166)
Key features and details
- Rabbit polyclonal to GSK3 beta (phospho S9)
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GSK3 beta (phospho S9) antibody
GSK3 beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GSK3 beta (phospho S9) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Zebrafish, Macaque monkey, Gorilla
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免疫原
Synthetic peptide corresponding to Human GSK3 beta aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab129226) -
ポジティブ･コントロール
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A549. It also gave a positive result in MCF7 cell line. This antibody gave a positive result in IHC in the following FFPE tissue: Human breast adenocarcinoma
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab107166の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (3) |
Use a concentration of 1 µg/ml.
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
ターゲット情報
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機能
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. -
組織特異性
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. -
細胞内局在
Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 790875 Cow
- Entrez Gene: 2932 Human
- Entrez Gene: 56637 Mouse
- Entrez Gene: 100126852 Pig
- Entrez Gene: 84027 Rat
- Entrez Gene: 100169938 Sheep
- Entrez Gene: 30654 Zebrafish
- Omim: 605004 Human
see all -
別名
- Glycogen Synthase Kinase 3 Beta antibody
- Glycogen synthase kinase-3 beta antibody
- GSK 3 beta antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-GSK3 beta (phospho S9) antibody (ab107166)
ab107166 staining GSK3 beta (phospho S9) in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab107166 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-GSK3 beta (phospho S9) antibody (ab107166)All lanes : Anti-GSK3 beta (phospho S9) antibody (ab107166) at 1 µg/ml (Milk blocked - 5%)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 125 kDa, 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta (phospho S9) antibody (ab107166)IHC image of GSK3 beta (phospho S9) staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107166, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab107166 は 19 報の論文で使用されています。
- Li Y et al. Suxiao Jiuxin Pill alleviates myocardial ischemia-reperfusion injury through the ALKBH5/GSK3β/mTOR pathway. Chin Med 18:31 (2023). PubMed: 36959603
- Zhao T et al. Qi-Dong-Huo-Xue-Yin balances the immune microenvironment to protect against LPS induced acute lung injury. Front Pharmacol 14:1200058 (2023). PubMed: 37292149
- Cui SS et al. Mucin1 induced trophoblast dysfunction in gestational diabetes mellitus via Wnt/β-catenin pathway. Biol Res 56:48 (2023). PubMed: 37608294
- Donison N et al. Upregulation of LRRK2 following traumatic brain injury does not directly phosphorylate Thr175 tau. Front Cell Neurosci 17:1272899 (2023). PubMed: 38026695
- Feng C et al. The Dopamine D1 Receptor Attenuates Titanium Particle-Induced Inhibition of Osteogenesis by Activating the Wnt Signaling Pathway. Mediators Inflamm 2023:6331650 (2023). PubMed: 36700172