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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-GSK3 alpha 抗体 [EP793Y] (ab40870)

Submit a review Submit a question References (14)
Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
  • Immunocytochemistry/ Immunofluorescence - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
  • Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
  • Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
  • Anti-GSK3 alpha antibody [EP793Y] (ab40870)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP793Y] to GSK3 alpha
  • Suitable for: ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

製品の概要

  • 製品名

    Anti-GSK3 alpha antibody [EP793Y]
    GSK3 alpha 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP793Y] to GSK3 alpha

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human GSK3 alpha aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: P49840

  • ポジティブ･コントロール

    • WB: HAP1, HEK293T, HepG2 and HeLa cell lysate. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab40870の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/200.
WB
1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
ICC/IF
1/200.
WB
1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

画像

  • Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
    Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
    All lanes : Anti-GSK3 alpha antibody [EP793Y] (ab40870) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : GSK3A knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab40870 observed at 51 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab40870 Anti-GSK3 alpha antibody [EP793Y] was shown to specifically react with GSK3 alpha in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266742 (knockout cell lysate ab257979) was used. Wild-type and GSK3 alpha knockout samples were subjected to SDS-PAGE. ab40870 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
    Immunocytochemistry/ Immunofluorescence - Anti-GSK3 alpha antibody [EP793Y] (ab40870)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling GSK3 alpha with purified ab40870 at 1/200 dilution (10 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 alpha antibody [EP793Y] (ab40870)

    ab4870 at 1/50 dilution, staining GSK3 alpha in Human breast carcinoma tissue by Immunohistochemistry, Paraffin embedded section.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
    Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: GSK3 alpha knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab40870 observed at 51 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab40870 was shown to specifically react with GSK3 alpha in wild-type HAP1 cells. No band was observed when GSK3 alpha knockout samples were examined. Wild-type and GSK3 alpha knockout samples were subjected to SDS-PAGE. ab40870 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/5000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
    Western blot - Anti-GSK3 alpha antibody [EP793Y] (ab40870)
    Anti-GSK3 alpha antibody [EP793Y] (ab40870) at 1/5000 dilution + 10ug HeLa cell lysate.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • Anti-GSK3 alpha antibody [EP793Y] (ab40870)
    Anti-GSK3 alpha antibody [EP793Y] (ab40870)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (14)

ab40870 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab40870 は 14 報の論文で使用されています。

  • Wen J  et al. Pharmacological suppression of glycogen synthase kinase-3 reactivates HIV-1 from latency via activating Wnt/β-catenin/TCF1 axis in CD4+ T cells. Emerg Microbes Infect 11:391-405 (2022). PubMed: 34985411
  • Wang S  et al. Relationship between long non-coding RNA PCAT-1 expression and gefitinib resistance in non-small-cell lung cancer cells. Respir Res 22:146 (2021). PubMed: 33980216
  • Bali SK  et al. Glycogen synthase kinase 3 alpha/beta deletion induces precocious growth plate remodeling in mice. J Mol Med (Berl) 99:831-844 (2021). PubMed: 33609145
  • Gartz M  et al. Cardiomyocyte-produced miR-339-5p mediates pathology in Duchenne muscular dystrophy cardiomyopathy. Hum Mol Genet 30:2347-2361 (2021). PubMed: 34270708
  • Yang L  et al. Effect of Neurorepair for Motor Functional Recovery Enhanced by Total Saponins From Trillium tschonoskii Maxim. Treatment in a Rat Model of Focal Ischemia. Front Pharmacol 12:763181 (2021). PubMed: 34955834
View all Publications for this product

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