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リコンビナントRabMAb

Anti-GSDMA 抗体 [EPR19858-116] - BSA and Azide free (ab245942)

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Immunoprecipitation - Anti-GSDMA antibody [EPR19858-116] - BSA and Azide free (ab245942)
  • Anti-GSDMA antibody [EPR19858-116] - BSA and Azide free (ab245942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19858-116] to GSDMA - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-GSDMA antibody [EPR19858-116] - BSA and Azide free
    GSDMA 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19858-116] to GSDMA - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IP: HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.

  • 特記事項

    ab245942 is the carrier-free version of ab232254.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR19858-116

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245942の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
IP
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Function: May promote pyroptosis (Probable). Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4,5)-bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown. Tissue specificity: Expressed predominantly in the gastrointestinal tract and, at a lower level, in the skin. Also detected in mammary gland. In the gastrointestinal tract, mainly expressed in differentiated cells, including the differentiated cell layer of esophagus and mucus-secreting pit cells of the gastric epithelium. Down-regulateded in gastric cancer cells. Similarity: Belongs to the gasdermin family. Domain: Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain.

  • 細胞内局在

    Cytoplasm, perinuclear region

  • 参照データベース

    • 別名

      • FKSG9 antibody
      • Gasdermin 1 antibody
      • Gasdermin A antibody
      • Gasdermin-1 antibody
      • Gasdermin-A antibody
      • GSDM antibody
      • GSDM1 antibody
      • GSDMA antibody
      • GSDMA_HUMAN antibody
      see all

    画像

    • Immunoprecipitation - Anti-GSDMA antibody [EPR19858-116] - BSA and Azide free (ab245942)
      Immunoprecipitation - Anti-GSDMA antibody [EPR19858-116] - BSA and Azide free (ab245942)

      GSDMA was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate with ab232254 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab232254 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

      Lane 1: HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate 10 µg (Input). 

      Lane 2: ab232254 IP in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab232254 in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

      The bands lower than 50 kDa are the degraded exogenous GSDMA protein.

      The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab232254).

    • Anti-GSDMA antibody [EPR19858-116] - BSA and Azide free (ab245942)
      Anti-GSDMA antibody [EPR19858-116] - BSA and Azide free (ab245942)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245942 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245942 は論文での使用が確認できていません。

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