Anti-GRWD1 抗体 (ab176815)
Key features and details
- Rabbit polyclonal to GRWD1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GRWD1 antibody
GRWD1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GRWD1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan
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免疫原
Synthetic peptide within Human GRWD1 aa 1-50. The exact sequence is proprietary. NP_113673.2
Sequence:MAARKGRRRT CETGEPMEAE SGDTSSEGPA QVYLPGRGPP LREGEELVMD
Database link: Q9BQ67 -
ポジティブ･コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab176815 was affinity purified using an epitope specific to GRWD1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176815の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 49 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 540739 Cow
- Entrez Gene: 83743 Human
- Omim: 610597 Human
- SwissProt: Q1JQD2 Cow
- SwissProt: Q9BQ67 Human
- Unigene: 400625 Human
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別名
- CDW4 antibody
- CUL4 and DDB1 associated WDR protein 4 antibody
- Glutamate rich WD repeat containing 1 antibody
see all
画像
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Western blot - Anti-GRWD1 antibody (ab176815)All lanes : Anti-GRWD1 antibody (ab176815) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-GRWD1 antibody (ab176815)Detection of GRWD1 by Western Blot of Immunprecipitate.
ab176815 at 0.1 µg/ml labeling GRWD1 in HeLa whole cell lysate immunoprecipitated using ab176815 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab176815 は論文での使用が確認できていません。
