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AB205531

Anti-GRSF1 抗体 [EPR16678]

Anti-GRSF1 antibody [EPR16678]

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(11 Publications)

Rabbit Recombinant Monoclonal GRSF1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

別名を表示する

G-rich sequence factor 1, GRSF-1, GRSF1

8 Images
Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (AB205531)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (AB205531)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GRSF1 with ab205531 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab205531 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-GRSF1 antibody [EPR16678] (AB205531)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GRSF1 antibody [EPR16678] (AB205531)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GRSF1 with ab205531 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC)at 1/500 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (AB205531)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRSF1 antibody [EPR16678] (AB205531)

Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling GRSF1 with ab205531 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab205531 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibodyat 1/1000 dilution.

Immunoprecipitation - Anti-GRSF1 antibody [EPR16678] (AB205531)
  • IP

Supplier Data

Immunoprecipitation - Anti-GRSF1 antibody [EPR16678] (AB205531)

GRSF1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab205531 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab205531 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HeLa whole cell lysate 10μg (Input).

Lane 2 : ab205531 IP in HeLa whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab205531 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-GRSF1 antibody [EPR16678] (ab205531)

Predicted band size: 53 kDa

false

Western blot - Anti-GRSF1 antibody [EPR16678] (AB205531)
  • WB

Supplier Data

Western blot - Anti-GRSF1 antibody [EPR16678] (AB205531)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa

false

Exposure time: 30s

Western blot - Anti-GRSF1 antibody [EPR16678] (AB205531)
  • WB

Supplier Data

Western blot - Anti-GRSF1 antibody [EPR16678] (AB205531)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa

false

Exposure time: 2s

Western blot - Anti-GRSF1 antibody [EPR16678] (AB205531)
  • WB

Supplier Data

Western blot - Anti-GRSF1 antibody [EPR16678] (AB205531)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse kidney lysate at 10 µg

Lane 3:

Rat brain lysate at 10 µg

Lane 4:

Rat heart lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa

false

Exposure time: 30s

Western blot - Anti-GRSF1 antibody [EPR16678] (AB205531)
  • WB

Supplier Data

Western blot - Anti-GRSF1 antibody [EPR16678] (AB205531)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GRSF1 antibody [EPR16678] (ab205531) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus ) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa

false

Exposure time: 30s

関連する標識済み抗体及び組成の異なる製品 (6)

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    Anti-GRSF1 antibody [EPR16678] - BSA and Azide free

  • 660 APC

    APC Anti-GRSF1 antibody [EPR16678]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-GRSF1 antibody [EPR16678]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-GRSF1 antibody [EPR16678]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-GRSF1 antibody [EPR16678]

  • 578 PE

    PE Anti-GRSF1 antibody [EPR16678]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16678

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB, IP, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB194358

Anti-GRSF1 antibody [EPR16679]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRSF1 antibody [EPR16679] (AB194358)

  • 4
  • 1

組合せ製品

Primary Antibodies

AB186735

Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)

  • 5
  • 16
Primary Antibodies

AB185942

Anti-MRPP3 antibody [EPR14321-30]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-MRPP3 antibody [EPR14321-30] (AB185942)

  • 0
  • 0
Primary Antibodies

AB240098

Anti-SDHA antibody [EPR9043(B)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-SDHA antibody [EPR9043(B)] - BSA and Azide free (AB240098)

  • 5
  • 2
Primary Antibodies

AB194826

Anti-MRPL48 antibody [EPR16328]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL48 antibody [EPR16328] (AB194826)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/150", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The G-rich RNA sequence binding factor 1 known as GRSF1 is a protein involved in RNA processing activities. Alternate names include MGC60760 and GAR1. This protein weighs approximately 48 kDa and demonstrates a wide expression pattern across different tissues with a marked presence in mitochondria. GRSF1 binds specifically to G-rich elements in RNA playing a mechanical role in stabilizing complex RNA structures and facilitating efficient RNA processing.
Biological function summary

The GRSF1 operates as an important player in mitochondrial RNA metabolism. It is known to interact within ribonucleoprotein complexes facilitating the regulation of mitochondrial gene expression. GRSF1 contributes to the maturation and processing of mitochondrial RNA impacting the proper synthesis of components essential for mitochondrial function. It ensures that mitochondrial encoded proteins are synthesized accurately aiding overall cellular energy production.

Pathways

GRSF1 engagement with organellar RNA processing integrates into mitochondrial biogenesis and maintenance pathways. This protein significantly influences the electron transport chain by ensuring the precise maturation of mitochondrial RNAs required for encoding electron transport chain components. It connects with proteins like LRPPRC as part of RNA granules coordinating gene expression regulation to support cellular respiration and energy conversion pathways.

GRSF1 links closely to conditions involving mitochondrial dysfunctions such as mitochondrial myopathy. Alterations in GRSF1 function can result in impaired energy production leading to muscle weakness and other systemic symptoms associated with myopathy. It may also intersect with neurodegenerative disorders where mitochondrial impairment represents a common pathological feature. GRSF1's association with proteins like Twinkle helicase in such diseases emphasizes its broader implications in maintaining mitochondrial integrity and cellular health.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences (PubMed : 23473033, PubMed : 23473034, PubMed : 29967381). Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PubMed : 29967381). Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome (PubMed : 29967381). G-quadruplexes (G4) are non-canonical 4 stranded structures formed by transcripts from the light strand of mtDNA (PubMed : 29967381).
See full target information G-rich sequence factor 1
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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40523799

2025

Transcription arrest induces formation of RNA granules in mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Katja G Hansen,Autum Baxter-Koenigs,Caroline Am Weiss,Erik McShane,L Stirling Churchman

Life science alliance 8: PubMed39622621

2024

Endo-bind-n-seq: identifying RNA motifs of RNA binding proteins isolated from endogenous sources.

Applications

Unspecified application

Species

Unspecified reactive species

Tiana Nicole Hanelt,Nora Treiber,Thomas Treiber,Gerhard Lehmann,Norbert Eichner,Tamara Rothmeier,Georg Schmid,Robert Reichelt,Federico Zambelli,Giulio Pavesi,Dina Grohmann,Gunter Meister

Journal of biochemical and molecular toxicology 38:e70062 PubMed39575578

2024

Mincle Maintains M1 Polarization of Macrophages and Contributes to Renal Aging Through the Syk/NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lingshuang Sun,Hua Liu,Kehui Shi,Meng Wei,Hongli Jiang

Cell death & disease 14:717 PubMed37923734

2023

GRSF1 antagonizes age-associated hypercoagulability via modulation of fibrinogen mRNA stability.

Applications

Unspecified application

Species

Unspecified reactive species

Doudou Liu,Chenzhong Xu,Ze Gong,Yijie Zhao,Zhiqiang Fang,Xiaoli Rao,Qingyu Chen,Guodong Li,Wei Kong,Jun Chen

Cell 186:3307-3324.e30 PubMed37385249

2023

Dynamic mapping of proteome trafficking within and between living cells by TransitID.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Qin,Joleen S Cheah,Charles Xu,James Messing,Brian D Freibaum,Steven Boeynaems,J Paul Taylor,Namrata D Udeshi,Steven A Carr,Alice Y Ting

Molecules (Basel, Switzerland) 28: PubMed37241859

2023

Salvianolic Acid B Inhibits Ferroptosis and Apoptosis during Myocardial Ischemia/Reperfusion Injury via Decreasing the Ubiquitin-Proteasome Degradation of GPX4 and the ROS-JNK/MAPK Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojin Xu,Chenhan Mao,Chengbo Zhang,Meng Zhang,Jianbin Gong,Xindong Wang

Frontiers in pharmacology 13:948128 PubMed36120303

2022

Xiao-Yao-San protects against anti-tuberculosis drug-induced liver injury by regulating Grsf1 in the mitochondrial oxidative stress pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zijun Bai,Weiwei Tao,Yiqun Zhou,Yi Cao,Shun Yu,Zheng Shi

Frontiers in pharmacology 13:879870 PubMed35600863

2022

Geniposide Possesses the Protective Effect on Myocardial Injury by Inhibiting Oxidative Stress and Ferroptosis Activation of the Grsf1/GPx4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuehong Shen,Xindong Wang,Xinyu Shen,Yue Wang,Shulin Wang,Yunyun Zhang,Xiaoming Yao,Yijiao Xu,Ming Sang,Jiamin Pan,Yu Qin,Qian Zhou,Jianping Shen

Nature communications 12:4980 PubMed34404792

2021

Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Qin,Samuel A Myers,Dominique K Carey,Steven A Carr,Alice Y Ting

Aging 13:14557-14570 PubMed34078750

2021

GRSF1 deficiency in skeletal muscle reduces endurance in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Riley K Driscoll,Linda K Krasniewski,Samuel G Cockey,Jen-Hao Yang,Yulan Piao,Elin Lehrmann,Yongqing Zhang,Marc Michel,Ji Heon Noh,Chang-Yi Cui,Myriam Gorospe
View all publications
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