Anti-GRP94 抗体 (ab13509)
Key features and details
- Rabbit polyclonal to GRP94
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GRP94 antibody
GRP94 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GRP94 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Mouse GRP94 aa 787-802 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:EEEEETEKESTEKDEL
Database link: P08113 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13509の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 94 kDa. 2µg/mL of ab13509 detects of Grp94 in 20µg of Rat Brain Tissue Extract using colorimetric analysis. 1/2000 for ECL detection.
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 94 kDa. 2µg/mL of ab13509 detects of Grp94 in 20µg of Rat Brain Tissue Extract using colorimetric analysis. 1/2000 for ECL detection. |
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ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. -
配列類似性
Belongs to the heat shock protein 90 family. -
細胞内局在
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 7184 Human
- Entrez Gene: 22027 Mouse
- Entrez Gene: 362862 Rat
- Omim: 191175 Human
- SwissProt: P14625 Human
- SwissProt: P08113 Mouse
- SwissProt: Q66HD0 Rat
- Unigene: 192374 Human
see all -
別名
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
see all
画像
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Western blot - Anti-GRP94 antibody (ab13509)All lanes : Anti-GRP94 antibody (ab13509) at 1/1000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Lysates prepared from rat brain
Lane 3 : Lysates prepared from mouse brain
Lane 4 : Lysates prepared from rat liver microsomes
Lane 5 : Lysates prepared from mouse liver microsomes
Lane 6 : Lysates prepared from human liver microsomes
Predicted band size: 94 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab13509)ICC/IF image of ab13509 stained rat PC12 cells. The cells were 4% PFA and 100% methanol fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13509, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). -
Western blot - Anti-GRP94 antibody (ab13509)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab13509 は 21 報の論文で使用されています。
- Meng Q et al. Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway. J Cell Mol Med 25:1867-1883 (2021). PubMed: 33417281
- Wang X et al. Identification of small extracellular vesicle subtypes in follicular fluid: Insights into the function and miRNA profiles. J Cell Physiol 236:5633-5645 (2021). PubMed: 33576507
- Lin PB et al. Moderate Ethanol Pre-treatment Mitigates ICH-Induced Injury via ER Stress Modulation in Rats. Front Mol Neurosci 14:682775 (2021). PubMed: 34248500
- Qu Y et al. Macrophage-derived extracellular vesicles regulates USP5-mediated HDAC2/NRF2 axis to ameliorate inflammatory pain. FASEB J 35:e21332 (2021). PubMed: 34423867
- Miraglia F et al. Alpha-Synuclein FRET Biosensors Reveal Early Alpha-Synuclein Aggregation in the Endoplasmic Reticulum. Life (Basel) 10:N/A (2020). PubMed: 32796544