Anti-GRP78 BiP 抗体 [EPR4041(2)]
Anti-GRP78 BiP antibody [EPR4041(2)]
- RabMAb
- Recombinant
- 詳細を見る
5
(3 Reviews)
|
(72 Publications)
Rabbit Recombinant Monoclonal GRP78 BiP antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 72 publications.
別名を表示する
GRP78, HSPA5, Endoplasmic reticulum chaperone BiP, 78 kDa glucose-regulated protein, Binding-immunoglobulin protein, Heat shock protein 70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein, GRP-78, BiP, HSP70 family protein 5
- WB
Lab
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
All lanes:
LnCaP at 20 µg
Secondary
All lanes:
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa
false
- WB
Lab
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
All lanes:
Raw264.7 cell lysate at 20 µg
Secondary
All lanes:
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 132 kDa,216 kDa,72 kDa
Observed band size: 160 kDa,170 kDa,208-216 kDa,78 kDa
false
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GRP78 BiP with purified ab108615 at 1/200 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Unpurified ab108615 at 1/50 dilution staining GRP78 BiP in Human breast carcinoma tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab108615 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
- WB
Lab
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/5000 dilution
All lanes:
HepG2 at 20 µg
Secondary
All lanes:
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa
false
- WB
Unknown
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
Lane 1:
HeLa cell lysates
Lane 2:
NIH/3T3 cell lysates
Lane 3:
LnCaP cell lysates
Lane 4:
HepG2 cell lysates
Secondary
All lanes:
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 72 kDa
false
- WB
Supplier Data
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
Lane 1:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2:
HeLa treated with 2.5 µg/ml tunicamycin for 24h whole cell lysates at 20 µg
Lane 3:
HUVEC (Human umbilical vein endothelial cell) whole cell lysates at 20 µg
Lane 4:
HUVEC treated with 10 µg/ml tunicamycin for 48h whole cell lysates at 20 µg
Lane 5:
Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg
Lane 6:
Raw 264.7 treated with 5 µg/ml tunicamycin for 18h whole cell lysates at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa
false
Exposure time: 3s
- WB
Lab
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Blocking/Diluting buffer and concentration : 5% NFDM/TBST
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
Lane 1:
MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2:
HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
Lane 3:
293T (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg
Lane 4:
U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 20 µg
Lane 5:
THP-1 (Human monocytic leukemia monocyte) whole cell lysates at 20 µg
Lane 6:
MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 7:
NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg
Lane 8:
Human uterus lysates at 20 µg
Lane 9:
Human liver lysates at 20 µg
Lane 10:
Mouse heart lysates at 20 µg
Lane 11:
Mouse lung lysates at 20 µg
Lane 12:
Mouse testis lysates at 20 µg
Lane 13:
Mouse liver lysates at 20 µg
Lane 14:
Rat liver lysates at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa
false
Exposure time: 26s
- WB
Lab
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Blocking and diluting buffer : 5% NFDM/TBST.
Tunicamycin induces the expression of GRP78 BiP reported by PMID 22859938
Exposure time :
Lanes 1 and 2 : 20 seconds
Lanes 3 and 4 : 30 seconds
Lanes 5 and 6 : 5 seconds
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
Lane 1:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2:
HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ug/mltunicamycin for 12 hours whole cell lysates at 15 µg
Lane 3:
MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 4:
MCF-7 (Human breast adenocarcinoma epithelial cell) treated with 10ug/mltunicamycin for 48 hours whole cell lysates at 15 µg
Lane 5:
MEF (Mouse embryonic fibroblast) whole cell lysates at 15 µg
Lane 6:
MEF (Mouse embryonic fibroblast) treated with 10ug/ml tunicamycin for 48 hours whole cell lysates at 15 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 72 kDa
false
関連する標識済み抗体及び組成の異なる製品 (9)
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Anti-GRP78 BiP antibody [EPR4041(2)] - BSA and Azide free
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-GRP78 BiP antibody [EPR4041(2)]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-GRP78 BiP antibody [EPR4041(2)]
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578 PE
PE Anti-GRP78 BiP antibody [EPR4041(2)]
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603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-GRP78 BiP antibody [EPR4041(2)]
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660 APC
APC Anti-GRP78 BiP antibody [EPR4041(2)]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-GRP78 BiP antibody [EPR4041(2)]
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-GRP78 BiP antibody [EPR4041(2)]
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775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-GRP78 BiP antibody [EPR4041(2)]
Reactivity data
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製品の詳細
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
出荷温度及び保存条件
製品の状態
精製方法
バッファー組成
出荷温度
短期保存期間
短期保存温度
長期保存温度
分注に関する情報
保管に関する情報
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GRP78/BiP is important in the unfolded protein response (UPR) a cellular stress response related to the ER. It is part of a complex that manages protein load inside the ER by regulating the fold of nascent proteins and interacting with other ER stress sensors like IRE1 PERK and ATF6. This function is essential for maintaining proper protein conformation in the ER especially during physiological stress ensuring that only correctly folded proteins proceed to the Golgi apparatus.
Pathways
GRP78/BiP significantly affects the UPR and apoptosis pathways. By controlling protein folding and quality in the ER GRP78 helps prevent cell death under stress conditions. It works closely with other proteins like ATF6 which activates stress response genes. In normal conditions GRP78 keeps stress transducers inactivated but during stress it dissociates allowing UPR signaling to occur.
製品プロトコール
- Visit the General protocols
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ターゲットの情報
文献 (72)
Recent publications for all applications. Explore the full list and refine your search
Inflammation 47:421-437 PubMed37898994
2023
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Journal of orthopaedic surgery and research 18:784 PubMed37853395
2023
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International journal of molecular sciences 24: PubMed37762310
2023
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Molecules (Basel, Switzerland) 28: PubMed37764275
2023
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Frontiers in nutrition 10:1192758 PubMed37583461
2023
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Communications biology 6:208 PubMed36813870
2023
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Neuropathology and applied neurobiology 49:e12877 PubMed36633103
2023
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Nature communications 13:2055 PubMed35440626
2022
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Asia-Pacific journal of clinical oncology 19:e27-e38 PubMed35394683
2022
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Molecular medicine reports 25: PubMed35419615
2022
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