Anti-Grp75/MOT 抗体 [9F8] (ab171089)

Immunofluorescence analysis of Grp75/MOT was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab171089 at 1:50 dilution in 0.1% BSA, incubated at 4ºC overnight and then labeled with Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

4-20% Tris-HCL polyacrylamide gel

Immunofluorescent analysis of HeLa cells labeling Grp75/MOT with ab171089 at 1/50 dilution (green) compared to negative control NIH3T3 cells. 

Detection of Grp75/MOT in Immunoprecipitates of HeLa whole cell lysates using ab171089 at 2µg per 750µg lysate. Detected with ab171089 at 1/1000 dilution.