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AB64943

Anti-GRK5 抗体

Anti-GRK5 antibody

5

(3 Reviews)

|

(7 Publications)

Rabbit Polyclonal GRK5 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human GRK5.

別名を表示する

GPRK5, GRK5, G protein-coupled receptor kinase 5, G protein-coupled receptor kinase GRK5

3 Images
Western blot - Anti-GRK5 antibody (AB64943)
  • WB

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Western blot - Anti-GRK5 antibody (AB64943)

All lanes:

Western blot - Anti-GRK5 antibody (ab64943) at 1/500 dilution

Lane 1:

HUVEC extract

Lane 2:

HepG2 cell extract

Lane 3:

HeLa cell extract

Lane 4:

HeLa cell extract with immunising peptide

Predicted band size: 67 kDa

Observed band size: 100 kDa,40 kDa,68 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-GRK5 antibody (AB64943)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GRK5 antibody (AB64943)

ICC/IF image of ab64943 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64943, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK5 antibody (AB64943)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK5 antibody (AB64943)

IHC image of ab64943 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64943, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Rat, Mouse

アプリケーション

ELISA, WB, ICC/IF, IHC-P, IHC-Fr

applications

免疫原

Synthetic Peptide within Human GRK5. The exact immunogen used to generate this antibody is proprietary information.

P34947

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Reactivity data

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GRK5 also known as G protein-coupled receptor kinase 5 plays an important role in the regulation of G protein-coupled receptors (GPCRs). This kinase phosphorylates activated receptors leading to desensitization and internalization. GRK5 has a molecular weight of approximately 68 kDa. Researchers find its expression in various tissues including heart brain and leukocytes with high levels in the heart. Its expression pattern suggests it has essential roles in cardiovascular and neural functions.
Biological function summary

The kinase modulates receptor signaling and is involved in regulating the sensitivity of cells to external signals. It is part of the GRK family which includes several kinases that modulate receptor signaling through phosphorylation. GRK5 interacts with plasma membrane receptors and can form part of larger signaling complexes. This interaction helps control processes such as cellular communication and response to environmental changes.

Pathways

GRK5 plays critical roles in signaling pathways like the beta-adrenergic signaling pathway and the regulation of blood pressure. It's closely related to other proteins such as GRK2 (also known as βARK1) which also participates in receptor desensitization. These pathways underline its involvement in cardiovascular regulation and adaptation to stimulus alterations. GRK5’s action in these pathways provides a balance in cellular responses to sustained signaling activity.

Alterations in GRK5 influence cardiovascular diseases including heart failure and hypertension. Changes in GRK5 expression or function can lead to improper regulation of blood pressure and heart rate demonstrating its significant role in maintaining cardiovascular health. Additionally the protein has links to neurodegenerative diseases such as Alzheimer’s where altered receptor activity and signaling imbalance occur. This kinase interacts with other proteins like β-amyloid precursor protein in neural tissues indicating its role in neural health and disease progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates : Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).
See full target information GRK5
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

JACC. Basic to translational science 7:366-380 PubMed35540100

2022

Genetic Catalytic Inactivation of GRK5 Impairs Cardiac Function in Mice Via Dysregulated P53 Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Marzano,Daniela Liccardo,Andrea Elia,Ines Mucio,Claudio de Lucia,Anna Maria Lucchese,Erhe Gao,Nicola Ferrara,Antonio Rapacciuolo,Nazareno Paolocci,Giuseppe Rengo,Walter J Koch,Alessandro Cannavo

Frontiers in cardiovascular medicine 8:719805 PubMed34901202

2021

Isoproterenol-Induced Cardiomyopathy Recovery Intervention: Amlexanox and Forskolin Enhances the Resolution of Catecholamine Stress-Induced Maladaptive Myocardial Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel Komla Adzika,Hongjian Hou,Adebayo Oluwafemi Adekunle,Ruqayya Rizvi,Joseph Adu-Amankwaah,Wenkang Shang,Kexue Li,Qi-Ming Deng,Richard Mprah,Marie Louise Ndzie Noah,Hong Sun

Frontiers in cell and developmental biology 9:719351 PubMed34631707

2021

Amlexanox and Forskolin Prevents Isoproterenol-Induced Cardiomyopathy by Subduing Cardiomyocyte Hypertrophy and Maladaptive Inflammatory Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel Komla Adzika,Hongjian Hou,Adebayo Oluwafemi Adekunle,Ruqayya Rizvi,Seyram Yao Adzraku,Kexue Li,Qi-Ming Deng,Richard Mprah,Marie Louise Ndzie Noah,Joseph Adu-Amankwaah,Jeremiah Ong'achwa Machuki,Wenkang Shang,Tongtong Ma,Stephane Koda,Xianluo Ma,Hong Sun

British journal of pharmacology 178:3498-3516 PubMed33844281

2021

β-Arrestin1 and GPCR kinase2 play permissive roles in Src-mediated endocytosis of α4β2 nicotinic ACh receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Srijan Acharya,Dooti Kundu,Kyeong-Man Kim

Neoplasma 66:261-270 PubMed30784280

2019

GRK5 promotes tumor progression in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

T L Zhao,X X Gan,Y Bao,W P Wang,B Liu,L H Wang

Schizophrenia research 159:130-5 PubMed25153362

2014

Increased G protein-coupled receptor kinase (GRK) expression in the anterior cingulate cortex in schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Adam J Funk,Vahram Haroutunian,James H Meador-Woodruff,Robert E McCullumsmith

Journal of neurochemistry 131:848-58 PubMed24995537

2014

Diet-induced obesity accelerates the onset of terminal phenotypes in α-synuclein transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Carola Rotermund,Felicia M Truckenmüller,Heinrich Schell,Philipp J Kahle
View all publications
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