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AB2776

Anti-GRK1 抗体 [D11]

Anti-GRK1 antibody [D11]

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(5 Publications)

Mouse Monoclonal GRK1 antibody. Suitable for ICC/IF and reacts with Mouse, Human, Cow samples. Cited in 5 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human GRK1.

別名を表示する

RHOK, GRK1, Rhodopsin kinase GRK1, RK, G protein-coupled receptor kinase 1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (AB2776)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (AB2776)

Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1 : 200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (AB2776)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (AB2776)

Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1 : 20) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (AB2776)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (AB2776)

Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1 : 200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (AB2776)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (AB2776)

Immunolocalization of GRK1 in bovine retina using ab2776.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

D11

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Cow, Human

アプリケーション

ICC/IF

applications

免疫原

Native Full Length Protein corresponding to Human GRK1.

Q15835

エピトープ

This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.

特異性

Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This antibody does not cross-react with other G protein-coupled kinases.

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代替製品

Primary Antibodies

AB108502

Anti-GRK1 antibody [EPR2039(2)]

RabMAb|Recombinant|Trial Size

Western blot - Anti-GRK1 antibody [EPR2039(2)] (AB108502)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB109303

Anti-GRK3 antibody [EPR2041(2)]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody [EPR2041(2)] (AB109303)

  • 0
  • 0
Primary Antibodies

AB244364

Anti-GRK6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK6 antibody (AB244364)

  • 0
  • 0
Primary Antibodies

AB64943

Anti-GRK5 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK5 antibody (AB64943)

  • 5
  • 3
Proteins & Peptides

AB125599

Recombinant human GRK7 protein (GST tag N-Terminus)

Functional Studies - Recombinant human GRK7 protein (AB125599)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Cow": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
バッファー組成
Preservative: 0.05% Sodium azide
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GRK1 also known as G-protein-coupled receptor kinase 1 or rhodopsin kinase is an enzyme with a molecular mass of approximately 63 kDa. It plays a critical role in the phosphorylation process of activated rhodopsin. This kinase is mainly expressed in the retina particularly in rod photoreceptor cells where it aids in the regulation of phototransduction by rapidly shutting off the rhodopsin signaling cycle.
Biological function summary

GRK1 functions as part of a coordinated mechanism that ensures proper desensitization and recovery of photoreceptors after they are exposed to light. It is instrumental in the phototransduction cascade preventing excessive activation by phosphorylating light-activated rhodopsin which then allows the binding of arrestin. This phosphorylation-arrestin binding step is part of a larger complex that maintains the sensitivity of photoreceptors to changing light conditions.

Pathways

GRK1 participates in the critical visual signal transduction pathway within the retina. It works in conjunction with proteins such as arrestin and rhodopsin to modulate the phototransduction cascade ensuring proper visual responses. This kinase interacts with these proteins to modulate their activity and help in adapting to varying light conditions emphasizing its importance in maintaining visual clarity.

GRK1 mutations are connected to conditions like Oguchi disease a type of congenital stationary night blindness. This disease arises when rhodopsin phosphorylation is disrupted leading to impaired vision adaptation in low light. GRK1 links to the arrestin protein in this context as both proteins are necessary for proper rod cell function and dysfunction in either can lead to similar visual disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade (PubMed : 15946941). This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity). May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).
See full target information GRK1
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 33:108360 PubMed33176149

2020

The Deubiquitinating Enzyme Ataxin-3 Regulates Ciliogenesis and Phagocytosis in the Retina.

Applications

Unspecified application

Species

Unspecified reactive species

Vasileios Toulis,Sílvia García-Monclús,Carlos de la Peña-Ramírez,Rodrigo Arenas-Galnares,Josep F Abril,Sokol V Todi,Naheed Khan,Alejandro Garanto,Maria do Carmo Costa,Gemma Marfany

The Journal of biological chemistry 294:19119-19136 PubMed31694913

2019

The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.

Applications

Unspecified application

Species

Unspecified reactive species

Poppy Datta,Brandon Hendrickson,Sarah Brendalen,Avri Ruffcorn,Seongjin Seo

The Journal of clinical investigation 126:3006-22 PubMed27400127

2016

Truncated netrin-1 contributes to pathological vascular permeability in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Khalil Miloudi,François Binet,Ariel Wilson,Agustin Cerani,Malika Oubaha,Catherine Menard,Sullivan Henriques,Gaelle Mawambo,Agnieszka Dejda,Phuong Trang Nguyen,Flavio A Rezende,Steve Bourgault,Timothy E Kennedy,Przemyslaw Sapieha

Investigative ophthalmology & visual science 52:6793-800 PubMed21474765

2011

Variation in rhodopsin kinase expression alters the dim flash response shut off and the light adaptation in rod photoreceptors.

Applications

WB

Species

Unspecified reactive species

Keisuke Sakurai,Joyce E Young,Vladimir J Kefalov,Shahrokh C Khani

The Journal of biological chemistry 281:19426-32 PubMed16675451

2006

Recoverin binds exclusively to an amphipathic peptide at the N terminus of rhodopsin kinase, inhibiting rhodopsin phosphorylation without affecting catalytic activity of the kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew K Higgins,Daniel D Oprian,Gebhard F X Schertler
View all publications
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