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Anti-GRC5 / PHF2 抗体 (ab65771)

Submit a review Q&A (1)
Western blot - Anti-GRC5 / PHF2 antibody - Carboxyterminal end (ab65771)

    Key features and details

    • Rabbit polyclonal to GRC5 / PHF2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-GRC5 / PHF2 antibody

    • 製品の詳細

      Rabbit polyclonal to GRC5 / PHF2

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide, corresponding to C terminal amino acids 830-1096 of Human GRC5/ PHF2

    • ポジティブ･コントロール

      • Nuclear extracts of HeLa cells.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab65771の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).
    特記事項
    WB
    1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).

    ターゲット情報

    • 機能

      Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.

    • 組織特異性

      Widely expressed, including in liver (at protein level).

    • 配列類似性

      Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
      Contains 1 JmjC domain.
      Contains 1 PHD-type zinc finger.

    • ドメイン

      The PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.

    • 翻訳後修飾

      Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.

    • 細胞内局在

      Nucleus > nucleolus.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CENP-35 antibody
        • GRC5 antibody
        • JHDM1E antibody
        • Jumonji C domain containing histone demethylase 1E antibody
        • Lysine-specific demethylase PHF2 antibody
        • PHD finger protein 2 antibody
        • Phf2 antibody
        • PHF2_HUMAN antibody
        see all

      画像

      参考文献 (0)

      ab65771 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab65771 は論文での使用が確認できていません。

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Question

      Hi,
      We are interested in purchasing a PHF2 antibody. Your antibody ab65771
      seems to be promising, but we have some questions about the peptide used
      to produce the antibody. The issues are, a 269 aa peptide is very very
      big for a synthetic peptide; is the information online correct? And,
      both human and mouse PHF2 have 1096 amino acids, while on the
      information on your webpage indicates the synthetic peptide used to
      generate the ab "corresponding to C terminal amino acids 830-1098 of
      Human GRC5 / PHF2". If possible please let us know if any of these
      information is incorrect, and provide us the correct information.
      Thank you,

      Read More
      Answer

      Thank you for contacting us and for your patience while I have been in touch with the lab regarding your enquiry.
      You are correct that there was a mistake on the datasheet. The immunogen runs from amino acid 830 to the C-terminus at amino acid 1096. It may be more accurate to call this a fragment than a peptide.
      I have updated the datasheet with the correct immunogen information, but please let me know if you have any further questions or if there is anything else that we can do for you.
      Have a nice day!

      Read More

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