Anti-GRB7 抗体 [EPR6858] (ab129091)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6858] to GRB7
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GRB7 antibody [EPR6858]
GRB7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6858] to GRB7 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- SKBR-3 whole cell lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6858 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129091の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
ターゲット情報
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機能
Interacts with the cytoplasmic domain of the epidermal growth factor receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds to ERBB2. -
配列類似性
Belongs to the GRB7/10/14 family.
Contains 1 PH domain.
Contains 1 Ras-associating domain.
Contains 1 SH2 domain. -
翻訳後修飾
Phosphorylated on tyrosine residues. - Information by UniProt
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参照データベース
- Entrez Gene: 2886 Human
- Omim: 601522 Human
- SwissProt: Q14451 Human
- Unigene: 86859 Human
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別名
- B47 antibody
- Epidermal growth factor receptor GRB 7 antibody
- Epidermal growth factor receptor GRB-7 antibody
see all
画像
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All lanes : Anti-GRB7 antibody [EPR6858] (ab129091) at 1/1000 dilution
Lanes 1 & 5 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 60 kDaExposure time: Lane 1-4: 180 seconds; Lane 5: 7 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The GRB7 expression level in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID 17916906 and 7907978.
This image is derived from purified batch.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab129091 は論文での使用が確認できていません。