Anti-GRB7 抗体 [EPR14099] (ab183737)
![Western blot - Anti-GRB7 antibody [EPR14099] (ab183737)](https://www.abcam.co.jp/ps/products/183/ab183737/Images/ab183737-392719-anti-grb7-antibody-epr14099-westernblot-human.jpg "Western blot - Anti-GRB7 antibody [EPR14099] (ab183737)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14099] to GRB7
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GRB7 antibody [EPR14099]
GRB7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14099] to GRB7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: SK-BR-3, T-47D, MCF7 and HepG2 cell lysates. IHC-P: Human breast carcinoma tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14099 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183737の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/50000. Predicted molecular weight: 60 kDa.
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000 - 1/50000. Predicted molecular weight: 60 kDa. |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Interacts with the cytoplasmic domain of the epidermal growth factor receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds to ERBB2. -
配列類似性
Belongs to the GRB7/10/14 family.
Contains 1 PH domain.
Contains 1 Ras-associating domain.
Contains 1 SH2 domain. -
翻訳後修飾
Phosphorylated on tyrosine residues. - Information by UniProt
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参照データベース
- Entrez Gene: 2886 Human
- Omim: 601522 Human
- SwissProt: Q14451 Human
- Unigene: 86859 Human
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別名
- B47 antibody
- Epidermal growth factor receptor GRB 7 antibody
- Epidermal growth factor receptor GRB-7 antibody
see all
画像
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Western blot - Anti-GRB7 antibody [EPR14099] (ab183737)Lane 1 : Anti-GRB7 antibody [EPR14099] (ab183737) at 1/1000 dilution (Purified)
Lanes 2-4 : Anti-GRB7 antibody [EPR14099] (ab183737) at 1/1000 dilution
Lane 1 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDaBlocking/Diluting buffer: 5% NFDM/TBST
The working pattern of ab183737 in SK-BR-3, T-47D and MCF7 is consistent with what has been described in PMID: 7907978 and 17916906
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR14099] (ab183737)](https://www.abcam.co.jp/ps/products/183/ab183737/Images/ab183737-4-anti-grb7-antibody-epr14099-immunohistochemistry-breast-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR14099] (ab183737)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling GRB7 with purified ab183737 at 1/500 dilution (0.82 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Anti-GRB7 antibody [EPR14099] (ab183737)](https://www.abcam.co.jp/ps/products/183/ab183737/Images/ab183737-6-benefits-of-recombinant-antibodies.png)
Anti-GRB7 antibody [EPR14099] (ab183737)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab183737 は 2 報の論文で使用されています。
- Saidy B et al. Unravelling transcriptomic complexity in breast cancer through modulation of DARPP-32 expression and signalling pathways. Sci Rep 13:21163 (2023). PubMed: 38036593
- Vermehren-Schmaedick A et al. The prognostic significance of GRB7 protein expression and localization in human breast and ovarian cancers. Oncotarget 11:2273-2289 (2020). PubMed: 32595827