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リコンビナントRabMAb

Anti-GRASP65 抗体 [EPR12439] - C-terminal (ab174834)

Reviews (4) Submit a question References (11)
Immunocytochemistry/ Immunofluorescence - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
  • Western blot - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
  • Flow Cytometry (Intracellular) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
  • Immunoprecipitation - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
  • Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12439] to GRASP65 - C-terminal
  • Suitable for: Flow Cyt (Intra), ICC/IF, IP, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-GRASP65 antibody [EPR12439] - C-terminal
    GRASP65 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12439] to GRASP65 - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, IP, IHC-P, WBmore details

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • 293T, HeLa, HepG2 and MCF7 cell lysates; Human colon and uterus tissues; HepG2 and HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR12439

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab174834の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (2)
1/1000 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 46 kDa).
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 46 kDa).

ターゲット情報

  • 機能

    Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.

  • 配列類似性

    Belongs to the GORASP family.
    Contains 1 PDZ (DHR) domain.

  • 翻訳後修飾

    Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher order oligomers, leading to cisternal unstacking.
    Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis.

  • 細胞内局在

    Golgi apparatus > cis-Golgi network membrane. Undergoes rapid exchange with the cytosol.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FLJ23443 antibody
      • Golgi peripheral membrane protein p65 antibody
      • Golgi phosphoprotein 5 antibody
      • Golgi reassembly and stacking protein 1 antibody
      • Golgi reassembly and stacking protein 65 kDa antibody
      • Golgi reassembly and stacking protein, 65-kD antibody
      • Golgi reassembly stacking protein 1 65kDa antibody
      • Golgi reassembly stacking protein 1 antibody
      • Golgi reassembly stacking protein of 65 kDa antibody
      • Golgi reassembly-stacking protein 1 antibody
      • Golgi reassembly-stacking protein of 65 kDa antibody
      • GOLPH 5 antibody
      • GOLPH5 antibody
      • Gorasp 1 antibody
      • GORASP1 antibody
      • GORS1_HUMAN antibody
      • GRASP 65 antibody
      • GRASP65 antibody
      • MGC118894 antibody
      • MGC118897 antibody
      • P65 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
      Immunocytochemistry/ Immunofluorescence - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

      Immunofluorescent analysis of HepG2 cells, labeling GRASP65 with ab174834 at 1/100 dilution (red). DAPI nuclear staining (blue).

    • Western blot - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
      Western blot - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
      All lanes : Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 46 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling GRASP65 with ab174834 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue, labeling GRASP65 with ab174834 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
      Flow Cytometry (Intracellular) - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

      Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling GRASP65with ab174834 at 1/10 dilution (red), or negative control rabbit IgG (green). 

       

       

    • Immunoprecipitation - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
      Immunoprecipitation - Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

      Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling GRASP65, immunoprecipitated using ab174834 at 1/10 dilution.

    • Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)
      Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834)

    参考文献 (11)

    ab174834 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab174834 は 11 報の論文で使用されています。

    • Chiu SC  et al. The PRMT5/HURP axis retards Golgi repositioning by stabilizing acetyl-tubulin and Golgi apparatus during cell migration. J Cell Physiol 237:1033-1043 (2022). PubMed: 34541678
    • Wei C  et al. RhoGEF Trio Regulates Radial Migration of Projection Neurons via Its Distinct Domains. Neurosci Bull 38:249-262 (2022). PubMed: 34914033
    • Yue X  et al. Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner. Commun Biol 4:1370 (2021). PubMed: 34876695
    • Nordgaard C  et al. Regulation of the Golgi Apparatus by p38 and JNK Kinases during Cellular Stress Responses. Int J Mol Sci 22:N/A (2021). PubMed: 34502507
    • Zhang Y  et al. Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging. Nat Methods 17:225-231 (2020). PubMed: 31907447
    View all Publications for this product

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-GRASP65 antibody [EPR12439] - C-terminal

     Average
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mice Hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    35 µg
    Specification
    Mice Hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 23 2022

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRASP65 antibody [EPR12439] - C-terminal

     Excellent
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    投稿 May 24 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRASP65 antibody [EPR12439] - C-terminal

     Excellent
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Rat hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    投稿 Nov 06 2017

    Western blot abreview for Anti-GRASP65 antibody [EPR12439] - C-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SU.86.86 (pancreatic carcinoma - liver metastasis))
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    30 µg
    Specification
    SU.86.86 (pancreatic carcinoma - liver metastasis)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    投稿 Oct 09 2015

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