Anti-Granzyme B 抗体 [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289895)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289895)](https://www.abcam.co.jp/ps/products/289/ab289895/Images/ab289895-483513-IHChumantonsil.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289895)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [EPR8260] to Granzyme B - Chimeric – BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra), IHC-P
- Reacts with: Human
製品の概要
-
製品名
Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free
Granzyme B 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [EPR8260] to Granzyme B - Chimeric – BSA and Azide free -
由来種
Rat -
アプリケーション
適用あり: WB, Flow Cyt (Intra), IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- WB: KARPAS-299 and SR whole cell lysates. IHC-P: Human tonsil and kidney tissue. Flow cyt: No-GFP-CD16.NK-92 cells.
-
特記事項
ab289895 is the carrier-free version of ab289888.
This rat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab134933). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Ion Exchange Chromatography -
ポリ/モノ
モノクローナル -
クローン名
EPR8260 -
アイソタイプ
IgG2a -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab289895の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
|
|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
配列類似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
-
参照データベース
- Entrez Gene: 3002 Human
- Omim: 123910 Human
- SwissProt: P10144 Human
- Unigene: 1051 Human
-
別名
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289895)
This data was developed using ab289888, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Granzyme B with ab289888 at 1/100 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human tonsil.
The section was incubated with ab289888 for 30 mins at room temperature and followed by rat IgG antibody (ab102248) for 8 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
-
![Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289895)](https://www.abcam.co.jp/ps/products/289/ab289895/Images/ab289895-483549-8260rat-FC.jpg)
Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289895)This data was developed using ab289888, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized No-GFP-CD16.NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells labelling Granzyme B with ab289888 at 1/50 dilution (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rat IgG Fc (DyLight 488, ab96971) at 1/2000 dilution was used as the secondary antibody.
-
![Western blot - Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289895)](https://www.abcam.co.jp/ps/products/289/ab289895/Images/ab289895-483511-8260MCArat.jpg)
Western blot - Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289895)All lanes : Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) (ab289888) at 1/1000 dilution
Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : SR (human pleural effusion lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 48 secondsThis data was developed using ab289888, the same antibody clone in a different buffer formulation.
Negative control: Jurkat (PMID:18437383, 25168906
5% NFDM/TBST was used as a blocking and diluting buffer.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289895)](https://www.abcam.co.jp/ps/products/289/ab289895/Images/ab289895-483547-EPR8260RtIgG2aYR092206PS100humankidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289895)This data was developed using ab289888, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Granzyme B with ab289888 at 1/100 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: no staining on the glomerulus and tubule epithelia in human kidney (PMID: 28900605).
The section was incubated with ab289888 for 30 mins at room temperature and followed by rat IgG antibody (ab102248) for 8 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used.
-
![Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289895)](https://www.abcam.co.jp/ps/products/289/ab289895/Images/ab289895-483551-Benefits-of-Recombinant-antibodies.jpg)
Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (Chimeric) - BSA and Azide free (ab289895)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab289895 は論文での使用が確認できていません。
