Anti-Granzyme B 抗体 (ab4059)
Key features and details
- Rabbit polyclonal to Granzyme B
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Granzyme B antibody
Granzyme B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Granzyme B -
由来種
Rabbit -
特異性
The immunogen used for this product shares 94% homology with Granzyme H. Cross-reactivity with this protein has not been confirmed experimentally.
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アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Pig, Common marmoset
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4059の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (20) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
配列類似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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参照データベース
- Entrez Gene: 3002 Human
- Entrez Gene: 14939 Mouse
- Entrez Gene: 100233184 Pig
- Entrez Gene: 171528 Rat
- Omim: 123910 Human
- SwissProt: P10144 Human
- SwissProt: P04187 Mouse
- SwissProt: P18291 Rat
see all -
別名
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (ab4059)Huszthy et al PLoS One. 2015 Aug 20;10(8):e0136089. doi: 10.1371/journal.pone.0136089. eCollection 2015. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Cytotoxic effector cells and regulatory T cells in human glioblastoma xenograft growing in immunocompetent rats.
Granzyme B-positive cytotoxic cells (ab4059 at a 1/75 dilution) in a GBM xenograft with rejection. Asterisks mark tissue lyzed by effector cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (ab4059)Human tonsil tissue stained with anti-Granzyme B antibody (ab4059).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (165)
ab4059 は 165 報の論文で使用されています。
- Harter MF et al. Analysis of off-tumour toxicities of T-cell-engaging bispecific antibodies via donor-matched intestinal organoids and tumouroids. Nat Biomed Eng 8:345-360 (2024). PubMed: 38114742
- Zhang M et al. Rapamycin improves Graves' orbitopathy by suppressing CD4+ cytotoxic T lymphocytes. JCI Insight 8:N/A (2023). PubMed: 36580373
- Sasaki M et al. Anti-tumor immunity enhancement by photodynamic therapy with talaporfin sodium and anti-programmed death 1 antibody. Mol Ther Oncolytics 28:118-131 (2023). PubMed: 36726602
- Savarin M et al. Tumor Radiosensitization by Gene Electrotransfer-Mediated Double Targeting of Tumor Vasculature. Int J Mol Sci 24:N/A (2023). PubMed: 36769077
- Lin Z et al. Targeting ZDHHC9 potentiates anti-programmed death-ligand 1 immunotherapy of pancreatic cancer by modifying the tumor microenvironment. Biomed Pharmacother 161:114567 (2023). PubMed: 36963362