AB4059

Anti-Granzyme B 抗体

Name: Anti-Granzyme B 抗体 (ab4059) | Abcam
Brand: Abcam Limited
SKU: AB4059
Price: 49000 JPY
Availability: InStock
Rating: 4 (22 reviews)

Anti-Granzyme B antibody

(22 Reviews)

(207 Publications)

Anti-Granzyme B antibody (ab4059) is a rabbit polyclonal antibody detecting Granzyme B in IHC-P. Suitable for Human.

- Over 160 publications
- Trusted since 2003

別名を表示する

CGL1, CSPB, CTLA1, GRB, GZMB, Granzyme B, C11, CTLA-1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, Granzyme-2, Human lymphocyte protein, SECT, T-cell serine protease 1-3E, CTSGL1, Lymphocyte protease, HLP

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (AB4059)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (AB4059)

Human tonsil tissue stained with anti-Granzyme B antibody (ab4059).

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (AB4059)

Cytotoxic effector cells and regulatory T cells in human glioblastoma xenograft growing in immunocompetent rats.

Granzyme B-positive cytotoxic cells (ab4059 at a 1/75 dilution) in a GBM xenograft with rejection. Asterisks mark tissue lyzed by effector cells.

Huszthy et al PLoS One. 2015 Aug 20;10(8):e0136089. doi: 10.1371/journal.pone.0136089. eCollection 2015. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

The immunogen used for this product shares 94% homology with Granzyme H. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-Granzyme B antibody (ab4059) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community
Anti-Granzyme B (ab4059) was first used in a scientific publication in 2003 and has been cited over 160 times in peer-reviewed journals.

Reviewed by scientists
Anti-Granzyme B (ab4059) has over 20 independent reviews from customers.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme B also known as GZMB GB11 or granzyme B protein is a serine protease with a molecular mass of approximately 32 kDa. It is expressed mainly in cytotoxic T lymphocytes and natural killer (NK) cells. This enzyme plays a mechanical role in inducing apoptosis in target cells serving as an effector protein in the immune system's defense against virally infected cells or transformed cancer cells. The activity of granzyme B relies on its ability to cleave after aspartate residues in substrate proteins leading to the activation of apoptotic pathways.

Biological function summary

Granzyme B participates prominently in the immune response by activating caspases particularly caspase-3 which promotes the breakdown of cellular components necessary for apoptosis. Granzyme B does not function in isolation but acts in concert with other immune system factors such as perforin to effectively induce cell death. Perforin creates pores in the target cell membrane allowing granzyme B to enter and instigate the apoptosis sequence. The enzyme also contributes to the processing of cytokines which enhances the immune response further.

Pathways

Studies have determined that granzyme B is critical in the apoptosis pathway particularly in the granule exocytosis pathway. It closely interacts with proteins such as perforin and other granzymes to mediate apoptosis in target cells. Granzyme B also plays a role in the inflammatory response and can influence pathways associated with cytotoxic T cell signaling. Its pathway interactions ensure effective elimination of damaged or infected cells maintaining tissue homeostasis.

Granzyme B has associations with autoimmune diseases and cancer. Abnormally high levels of granzyme B can contribute to tissue damage and inflammation in autoimmune conditions like rheumatoid arthritis. In the context of cancer granzyme B aids in tumor surveillance and destruction when functioning correctly but impaired granzyme B activity can lead to evasion of immune detection by cancerous cells. Perforin also plays a role in these conditions closely working with granzyme B to either protect against or drive disease progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 1985927, PubMed : 3262682, PubMed : 3263427). It cleaves after Asp (PubMed : 1985927, PubMed : 8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed : 31953257, PubMed : 32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity).

See full target information GZMB

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文献 (207)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8478 PubMed41006211

2025

CD137L promotes immune surveillance in melanoma via HLTF regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Long Liang,Lin Zhu,Xin Li,Wenbin Zhou,Yanbin Zhang,Mien-Chie Hung,Guanxiong Zhang,Yong Chen,Xinwei Kuang,Juan Su,Jing Liu,Xiang Chen,Hong Liu

Nature communications 16:8171 PubMed40890191

2025

AEBP1 drives fibroblast-mediated T cell dysfunction in tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Wang,Jie Li,Daqiang Song,Yushen Wu,Jiazhou Liu,Ziying Yi,Jiazheng Sun,Jiefeng Huang,Linling Wu,Xiang Zhang,Jingyuan Wan,Li Zhang,Chong Li,Fan Li,Yuxian Wei,Yong Zhu,Huimin Du,Guosheng Ren,Hongzhong Li

Journal of extracellular biology 4:e70080 PubMed40843441

2025

Surface-Engineered Natural Killer Cell-Derived Small Extracellular Vesicles Induce Potent Anti-Tumour Effects in Lung Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sung-Min Kang,Dokyung Jung,Soojeong Noh,Sanghee Shin,Minju Kim,Hanchae Cho,Byungheon Lee,Kyungmoo Yea,Moon-Chang Baek

British journal of cancer 133:582-593 PubMed40579444

2025

PARP inhibitor-induced anti-tumour chemokine response is suppressed by dipeptidyl peptidase 4 (DPP4) in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Christoph Stange,Tobias F Dreyer,Maximilian Riedel,Franziska Elsen,Stefanie Seitz,Dorine Hamann,Marion Kiechle,Holger Bronger

Journal of nanobiotechnology 23:405 PubMed40452005

2025

Fucoidan-decorated metal-zoledronic acid nanocomplexes suppress tumor metastasis by inducing ferroptotic cell death and enhancing cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Hsin-Ting Tsai,Chi Lin,Chu-Hung Chung,Wen-Jing Hsu,Ming-Yi Hsieh,Ming-Cheng Chiang,Tzu-Wei Lu,Fwu-Long Mi,Cheng-Wei Lin

Nature neuroscience 28:1160-1173 PubMed40404995

2025

Microglia activation orchestrates CXCL10-mediated CD8 T cell recruitment to promote aging-related white matter degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Janos Groh,Ruoqing Feng,Xidi Yuan,Lu Liu,Dennis Klein,Gladis Hutahaean,Elisabeth Butz,Zhen Wang,Lisa Steinbrecher,Jonas Neher,Rudolf Martini,Mikael Simons

NPJ vaccines 10:80 PubMed40258806

2025

Targeting B7-H3 enhances the efficacy of neoantigen-based cancer vaccine in combination with radiotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Tao-Wei Ke,Chia-Yi Chen,William Tzu-Liang Chen,Yuan-Yao Tsai,Shu-Fen Chiang,Chi-Hsien Huang,Yu-Sen Lin,Te-Hong Chen,Tsung-Wei Chen,Ji-An Liang,K S Clifford Chao,Kevin Chih-Yang Huang

Nature genetics 57:680-693 PubMed40069506

2025

Alterations in PD-L1 succinylation shape anti-tumor immune responses in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Long Liang,Xinwei Kuang,Yi He,Lin Zhu,Poyee Lau,Xin Li,Dingan Luo,Lan Gong,Wenbin Zhou,Fanglin Zhang,Xiaowei Liang,Zhuofeng Li,Bin Hu,Dandan Liu,Tao Ding,Hui Li,Shuang Zhao,Juan Su,Mien-Chie Hung,Jing Liu,Hong Liu,Xiang Chen

Cell death & disease 16:158 PubMed40050608

2025

PRMT3 drives PD-L1-mediated immune escape through activating PDHK1-regulated glycolysis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Hong Ding,Fang-Zhi Yan,Bo-Nan Xu,Hui Qian,Xia-Lu Hong,Shu-Qing Liu,Yuan-Yuan Luo,Si-Han Wu,Ling-Yan Cai,Xin Zhang,Wei-Fen Xie

Cancer immunology, immunotherapy : CII 74:103 PubMed39904884

2025

Pre-injection of exosomes can significantly suppress ovarian cancer growth by activating the immune system in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wang,Changyi Zhang,Huimin Zeng,Liangliang Wang,Zanhong Wang,Zhiqiang Han

View all publications

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