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Anti-Granulocytes 抗体 [HIS48] (ab33760)

Submit a review Q&A (4)References (7)

Key features and details

  • Mouse monoclonal [HIS48] to Granulocytes
  • Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Rat
  • Isotype: IgM

製品の概要

  • 製品名

    Anti-Granulocytes antibody [HIS48]
    Granulocytes 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [HIS48] to Granulocytes

  • 由来種

    Mouse

  • 特異性

    ab3760 recognises granulocytes and erythroid cells.

  • アプリケーション

    適用あり: IHC-Fr, IHC-P, ICC/IF, Flow Cytmore details

  • 種交差性

    交差種: Rat

  • 免疫原

    Tissue, cells or virus corresponding to Rat Granulocytes. PVG rat spleen cell suspension.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 8% Fetal calf serum, 3.16% Tris HCl
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    HIS48

  • アイソタイプ

    IgM

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab33760の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性

    Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.

参考文献 (7)

ab33760 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab33760 は 7 報の論文で使用されています。

  • Bao L  et al. Engineered neutrophil apoptotic bodies ameliorate myocardial infarction by promoting macrophage efferocytosis and inflammation resolution. Bioact Mater 9:183-197 (2022). PubMed: 34820565
  • Cerullo D  et al. Add-On Cyclic Angiotensin-(1-7) with Cyclophosphamide Arrests Progressive Kidney Disease in Rats with ANCA Associated Glomerulonephritis. Cells 11:N/A (2022). PubMed: 35954280
  • Markworth JF  et al. Local shifts in inflammatory and resolving lipid mediators in response to tendon overuse. FASEB J 35:e21655 (2021). PubMed: 34042218
  • Cerullo D  et al. Characterization of a Rat Model of Myeloperoxidase-Anti-Neutrophil Cytoplasmic Antibody-Associated Crescentic Glomerulonephritis. Nephron 145:428-444 (2021). PubMed: 33910203
  • Büttner P  et al. Arginine metabolism and nitric oxide turnover in the ZSF1 animal model for heart failure with preserved ejection fraction. Sci Rep 11:20684 (2021). PubMed: 34667218
  • Markworth JF  et al. Resolvin D1 supports skeletal myofiber regeneration via actions on myeloid and muscle stem cells. JCI Insight 5:N/A (2020). PubMed: 32750044
  • Ma S  et al. Caloric Restriction Reprograms the Single-Cell Transcriptional Landscape of Rattus Norvegicus Aging. Cell 180:984-1001.e22 (2020). PubMed: 32109414

レビューと Q&A

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レビューを送る 質問を送る

1-4 of 4 Abreviews or Q&A

Question

I need to ask you some questions about antibody ab33760
What epitop are this antibodies specific to? In other words, what proteins/receptors has activated neutrophils, which are staninig by this antybody, It's very important for us because we try to find difference between intact and activated cells.

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Answer

Thank you for contacting us.

The immunogen used for this antibody was not a specific peptide but rather a cell suspension from rat spleen. Unfortunately, we have not performed any detailed mapping on this clone and thus we currently do not have any empirical data to indicate which specific region or sequence is detected by this clone.

I am very sorry that on this occasion I could not be more helpful. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

, You mention that the ab33760 is specifically designed against neutrophil and rbc. I would like to know how can you be sure that the ab33760 does not recognize the same antigen present on monocyte or macrophage? Thank you

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Answer

Thank you for your inquiry. According to our records, the specificity of this clone is supported by the following papers:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1783230/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793189/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435159/

I hope this information helps

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Question

Dear John Thank you for your answer. Sorry, I think that my previous question wasn't right. It doesn't matter what sequence it is. I try to understand why this antibody is specific only for granulocytes?

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Answer

Thank you for your reply. I am sorry that I misunderstood your original email. The antibody ab33760 was generated from PVG rat spleen cell suspension and is specific to granulocytes and erythroid cells. Unfortunately I do not have any more information on the specificity of this antibody. If there is anything else I can help you with, please let me know.

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Question

Hello We often buy production of your firm and are very happy with received results but now I need to ask you some questions about antibody ab33760 What epitop are this antibodies specific to?

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Answer

Thank you for contacting Abcam. The antibody ab33760, anti-Granulocytes antibody [HIS48] was generated from PVG rat spleen cell suspensions. The exact epitope sequence is proprietary and I do not have access to that information and therefore I cannot tell you the exact sequence. I am sorry that I am unable to assist you further in this matter but if there is anything else I can help you with, please let me know.

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