AB187070

Anti-Granulin 抗体 [EPR18539-59]

Anti-Granulin antibody [EPR18539-59]

20ul selling size
RabMAb
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal Granulin antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse samples. Cited in 8 publications.

別名を表示する

Progranulin, PGRN, Acrogranin, Epithelin/granulin precursor, Glycoprotein of 88 Kda, PC cell-derived growth factor, Proepithelin, GP88, Glycoprotein 88, PCDGF, PEPI, Grn

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Granulin antibody [EPR18539-59] (AB187070)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Granulin antibody [EPR18539-59] (AB187070)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte macrophage cell line) cells labeling Granulin with ab187070 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on J774A.1 cell line.

The nuclear counter statin is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Negative control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-Granulin antibody [EPR18539-59] (AB187070)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Granulin antibody [EPR18539-59] (AB187070)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) cell line labeling Granulin with ab187070 at 1/1000 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-Granulin antibody [EPR18539-59] (AB187070)

Granulin was immunoprecipitated from 0.35 mg of J774A.1 (mouse reticulum cell sarcoma monocyte macrophage cell line) whole cell lysate with ab187070 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187070 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : J774A.1 lysate 10 μg (Input).

Lane 2 : ab187070 IP in J774A.1 lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab187070 in J744A.1 whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Granulin antibody [EPR18539-59] (ab187070)

Predicted band size: 64 kDa

Observed band size: 88 kDa

true

Supplier Data

Western blot - Anti-Granulin antibody [EPR18539-59] (AB187070)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lane 1 : 3 seconds.

Lane 2 : 10 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID : 23972823). Granulin (Progranulin, PGRN) is a 593-amino acid, 68.5-kDa cysteine-rich protein that is typically secreted in a highly glycosylated 88-kDa form.

Lane 1:

Western blot - Anti-Granulin antibody [EPR18539-59] (ab187070) at 1/1000 dilution

Lane 2:

Western blot - Anti-Granulin antibody [EPR18539-59] (ab187070) at 1/5000 dilution

Lane 1:

J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage cell line) whole cell lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 64 kDa

Observed band size: 88 kDa

true

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR18539-59

アイソタイプ

IgG

キャリアフリー

交差種

Mouse

アプリケーション

Flow Cyt (Intra), ICC/IF, WB, IHC-P, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" } } }

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製品の詳細

ab187070 is also recommended for human and rat species for IHC.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Granulin also known as progranulin or GRN is a protein with a molecular mass of approximately 68 kDa. This protein is encoded by the GRN gene and is involved in various cellular processes. Granulin is expressed widely within the body particularly in epithelial cells immune cells and neurons. Researchers have developed methods like GRN ELISA and progranulin ELISA to quantitatively measure the levels of granulin in biological samples aiding in further understanding of its role in health and disease.

Biological function summary

Granulin functions as a growth factor involved in tissue repair cell proliferation and inflammation. It does not function as part of a larger protein complex but acts individually in exerting its effects on cells. Granulin influences various aspects of cellular architecture and signaling pathways making it important for maintaining cellular homeostasis. Additionally the granulin protein participates in regulating networks that involve cytokines and growth factors impacting both the innate and adaptive immune responses.

Pathways

Granulin plays significant roles in the MAPK and NF-kB signaling pathways. These pathways critically manage cellular reactions to stress inflammation and other external stimuli. Granulin modulates these processes often interacting with proteins like TNF receptors and interleukins to affect the downstream signaling events. Interactions between granulin and other signaling molecules allow it to integrate into broader networks that oversee various biological responses.

Significant links exist between granulin mutations and frontotemporal dementia (FTD) and Alzheimer's disease. Mutations in the GRN gene often lead to reduced granulin levels contributing to neurodegenerative conditions. These mutations affect the protein's normal function promoting pathological mechanisms associated with neuronal deterioration. Granulin interacts with proteins such as TDP-43 in neurodegenerative diseases where alterations might result in protein aggregation and neurotoxicity. Understanding these connections can lead to potential therapeutic interventions targeting granulin-related pathways.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed : 12524533, PubMed : 20026663, PubMed : 23041626, PubMed : 27789271, PubMed : 28073925, PubMed : 28453791, PubMed : 28541286, PubMed : 8496151). Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes (PubMed : 27789271, PubMed : 28453791, PubMed : 28541286). Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed : 28073925). In addition, functions as a wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (PubMed : 12524533). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed : 8496151). Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (PubMed : 20026663, PubMed : 23041626).. Granulin-3. Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8.. Granulin-7. Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.

See full target information Grn

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:28482 PubMed40759713

2025

The inhibitory effect of M2 macrophage-derived exosomes on gefitinib resistant lung adenocarcinoma cells through the MIF/TIMP1/CD74 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingcui Peng,Yan Zhang,Bin Li,Xin He,Cuimin Ding,Wenxia Hu

Nature communications 16:5621 PubMed40595567

2025

Connexin43 hemichannel blockade turns microglia neuroprotective and mitigates cognitive deficits in a mouse model of amyloidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yixun Su,Hui Li,Wenjie Zhang,Shi Tao,Qi Wang,Xuan Zhang,Mi Zhou,Xiaomin Huang,Chenmeng Wang,Yong Tang,Hui Chen,Alexei Verkhratsky,Zhengbao Zha,Jianqin Niu,Chenju Yi

Nature communications 14:6885 PubMed37898598

2023

The Lin28b/Wnt5a axis drives pancreas cancer through crosstalk between cancer associated fibroblasts and tumor epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoqi Shu,Minghe Fan,Bo Tu,Zhiheng Tang,Haojie Wang,Haimeng Li,Hengchao Li,Meng Yuan,Jingru Bai,Sihan Huo,Lina Wang,Wei-Guo Zhu,Wei Wang,Xiaoyun Liu,Shaokun Shu,Ying Zhao

Nature communications 14:5804 PubMed37726325

2023

Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Yachao He,Ibrahim Kaya,Reza Shariatgorji,Johan Lundkvist,Lars U Wahlberg,Anna Nilsson,Dejan Mamula,Jan Kehr,Justyna Zareba-Paslawska,Henrik Biverstål,Karima Chergui,Xiaoqun Zhang,Per E Andren,Per Svenningsson

Frontiers in molecular neuroscience 16:1170313 PubMed37138705

2023

Peroxisomal defects in microglial cells induce a disease-associated microglial signature.

Applications

Unspecified application

Species

Unspecified reactive species

Quentin Raas,Ali Tawbeh,Mounia Tahri-Joutey,Catherine Gondcaille,Céline Keime,Romain Kaiser,Doriane Trompier,Boubker Nasser,Valerio Leoni,Emma Bellanger,Maud Boussand,Yannick Hamon,Alexandre Benani,Francesca Di Cara,Caroline Truntzer,Mustapha Cherkaoui-Malki,Pierre Andreoletti,Stéphane Savary

International journal of molecular sciences 23: PubMed36233357

2022

Decreased Prosaposin and Progranulin in the Cingulate Cortex Are Associated with Schizophrenia Pathophysiology.

Applications

Unspecified application

Species

Unspecified reactive species

Yachao He,Xiaoqun Zhang,Ivana Flais,Per Svenningsson

Journal of experimental & clinical cancer research 40:4 PubMed33390170

2021

Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8 T cell exclusion.

Applications

Species

Mouse

Wenli Fang,Ting Zhou,He Shi,Mengli Yao,Dian Zhang,Husun Qian,Qian Zeng,Yange Wang,Fangfang Jin,Chengsen Chai,Tingmei Chen

Molecular medicine reports 22:3482-3492 PubMed32945448

2020

Abnormal angiogenesis of placenta in progranulin‑deficient mice.

Applications

WB, IHC-P

Species

Mouse, Mouse

Bairuo Xu,Xingyou Chen,Yubin Ding,Chang Chen,Taihang Liu,Hua Zhang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Granulin 抗体 [EPR18539-59]

Anti-Granulin antibody [EPR18539-59]

Immunocytochemistry/ Immunofluorescence - Anti-Granulin antibody [EPR18539-59] (AB187070)

Flow Cytometry (Intracellular) - Anti-Granulin antibody [EPR18539-59] (AB187070)

Immunoprecipitation - Anti-Granulin antibody [EPR18539-59] (AB187070)

Western blot - Anti-Granulin antibody [EPR18539-59] (AB187070)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (8)

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Abcam product promise