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AB169325

Anti-Granulin 抗体

Anti-Granulin antibody

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(1 Publication)

Mouse Polyclonal Granulin antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GRN.

別名を表示する

Progranulin, PGRN, Acrogranin, Epithelin precursor, Glycoprotein of 88 Kda, Granulin precursor, PC cell-derived growth factor, Proepithelin, GP88, Glycoprotein 88, PCDGF, PEPI, GRN

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Granulin antibody (AB169325)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Granulin antibody (AB169325)

Immunofluorescent analysis of permeabilized HeLa cells labeling Granulin with purified ab169325 at 10μg/ml.
Antibody purification with Protein A will be needed prior to use.

Western blot - Anti-Granulin antibody (AB169325)
  • WB

Unknown

Western blot - Anti-Granulin antibody (AB169325)

All lanes:

Western blot - Anti-Granulin antibody (ab169325) at 1/500 dilution

Lane 1:

Granulin transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 37 kDa,64 kDa

true

Key facts

宿主種

Mouse

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

Recombinant Full Length Protein corresponding to Human GRN.

P28799

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p>Antibody purification with Protein A will be needed prior to use.</p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製度
Whole antiserum
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Granulin also known as progranulin or GRN is a protein with a molecular mass of approximately 68 kDa. This protein is encoded by the GRN gene and is involved in various cellular processes. Granulin is expressed widely within the body particularly in epithelial cells immune cells and neurons. Researchers have developed methods like GRN ELISA and progranulin ELISA to quantitatively measure the levels of granulin in biological samples aiding in further understanding of its role in health and disease.
Biological function summary

Granulin functions as a growth factor involved in tissue repair cell proliferation and inflammation. It does not function as part of a larger protein complex but acts individually in exerting its effects on cells. Granulin influences various aspects of cellular architecture and signaling pathways making it important for maintaining cellular homeostasis. Additionally the granulin protein participates in regulating networks that involve cytokines and growth factors impacting both the innate and adaptive immune responses.

Pathways

Granulin plays significant roles in the MAPK and NF-kB signaling pathways. These pathways critically manage cellular reactions to stress inflammation and other external stimuli. Granulin modulates these processes often interacting with proteins like TNF receptors and interleukins to affect the downstream signaling events. Interactions between granulin and other signaling molecules allow it to integrate into broader networks that oversee various biological responses.

Significant links exist between granulin mutations and frontotemporal dementia (FTD) and Alzheimer's disease. Mutations in the GRN gene often lead to reduced granulin levels contributing to neurodegenerative conditions. These mutations affect the protein's normal function promoting pathological mechanisms associated with neuronal deterioration. Granulin interacts with proteins such as TDP-43 in neurodegenerative diseases where alterations might result in protein aggregation and neurotoxicity. Understanding these connections can lead to potential therapeutic interventions targeting granulin-related pathways.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

The protein expressed by the GRN gene is a secreted regulator of lysosomal function and a growth factor involved in processes such as inflammation, wound healing, and cell proliferation. It regulates protein trafficking to lysosomes and lysosomal enzyme activity, facilitating lysosomal acidification which leads to the degradation of mature CTSD by CTSB. It also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation, thus preventing the release of oxidants and proteases. Additionally, it modulates inflammation in neurons by preserving neuron survival, axonal outgrowth, and neuronal integrity. Granulin-3, -4, and -7 have distinct roles : Granulin-4 promotes proliferation of epithelial cell line A431 in culture; Granulin-3 inhibits epithelial cell proliferation and induces IL-8 secretion; and Granulin-7 stabilizes CTSD by maintaining its aspartic-type peptidase activity through interaction. This supplementary information is collated from multiple sources and compiled automatically.
See full target information GRN
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38892211

2024

Inflammatory and Cardiovascular Biomarkers to Monitor Fabry Disease Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Adrián Alonso-Núñez,Tania Pérez-Márquez,Marta Alves-Villar,Carlos Fernández-Pereira,Julián Fernández-Martín,Alberto Rivera-Gallego,Cristina Melcón-Crespo,Beatriz San Millán-Tejado,Aurora Ruz-Zafra,Remedios Garofano-López,Rosario Sánchez-Martínez,Elena García-Payá,Manuel López-Mendoza,Ignacio Martín-Suárez,Saida Ortolano
View all publications
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代替製品

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Western blot - Anti-Granulin antibody (AB191211)

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Primary Antibodies

AB227816

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  • 0
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Primary Antibodies

AB208777

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  • 0
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Primary Antibodies

AB225532

Anti-Granulin antibody [EPR15864] - Low endotoxin, Azide free

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granulin antibody [EPR15864] - Low endotoxin, Azide free (AB225532)

  • 0
  • 0

組合せ製品

Assay Kits

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Human Progranulin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB171941

Anti-FGF21 antibody [EPR8314(2)]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-FGF21 antibody [EPR8314(2)] (AB171941)

  • 3
  • 1
Primary Antibodies

AB181053

Anti-G-CSF antibody [EPR3203(N)(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (AB181053)

  • 4
  • 1

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