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リコンビナントRabMAb

Anti-GPVI 抗体 [EPR25283-14] - BSA and Azide free (ab289987)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
  • Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
  • Flow Cytometry - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
  • Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
  • Immunoprecipitation - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
  • Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
  • Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
  • Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25283-14] to GPVI - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IHC-Fr, IP
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-GPVI antibody [EPR25283-14] - BSA and Azide free
    GPVI 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25283-14] to GPVI - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, Flow Cyt, IHC-Fr, IPmore details

  • 種交差性

    交差種: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse platelet lysate. IHC-P: Mouse spleen tissue. IHC-Fr: Mouse spleen (fresh) tissue. Flow cyt: Mouse blood cells. IP: Mouse platelet lysate.

  • 特記事項

    ab289987 is the carrier-free version of ab289964.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.2
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR25283-14

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289987の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 37 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 37 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Collagen receptor involved in collagen-induced platelet adhesion and activation. Plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcR gamma-chain, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2.

  • 組織特異性

    Megakaryocytes and platelets.

  • 関連疾患

    Bleeding disorder, platelet-type 11

  • 配列類似性

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

  • 翻訳後修飾

    N-linked glycosylation at Asn-92 is not required for the cell surface expression, but contributes to maximal adhesion to type I collagen, collagen-related peptide (CRP), and, to a lesser extent, to the snake venom C-type lectin convulxin (CVX).

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Glycoprotein 6 antibody
      • Glycoprotein VI antibody
      • GP6 antibody
      • GPIV antibody
      • GPVI antibody
      • GPVI_HUMAN antibody
      • MGC138168 antibody
      • Platelet collagen receptor antibody
      • Platelet glycoprotein VI antibody
      • Platelet glycoprotein VI precursor antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)

      This data was developed using ab289964, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling GPVI with ab289964 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplasmic staining on megakaryocytes and platelets of mouse spleen. The section was incubated with ab289964 at 4°C overnight. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

      Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

    • Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
      Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)

      This data was developed using ab289964, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling GPVI with ab289964 at 1/100 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Positive staining in megakaryocytes of mouse spleen is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    • Flow Cytometry - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
      Flow Cytometry - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)

      This data was developed using ab289964, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Mouse blood cells cells labelling GPVI with ab289964 at 1/500 dilution (0.1µg)(Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) was used as secondary antibody at 1/2000 dilution. Cells were stained with rabbit IgG or ab289964. Then stained with anti-CD41 conjµgated to APC. Gated on viable cells.

    • Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
      Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
      All lanes : Anti-GPVI antibody [EPR25283-14] (ab289964) at 1/1000 dilution

      Lane 1 : Mouse platelet lysate
      Lane 2 : Mouse cerebellum tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

      Predicted band size: 37 kDa
      Observed band size: 58 kDa why is the actual band size different from the predicted?



      This data was developed using 289964, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The expression profile/ molecular weight  observed is consistent with what has been described in the literature (PMID: 23112346, 16014566).

      Negative control: cerebellum. (PMID: 10961879).

      Exposure time: 70 seconds.

    • Immunoprecipitation - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
      Immunoprecipitation - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)

      This data was developed using ab289964, the same antibody clone in a different buffer formulation.

      GPVI was immunoprecipitated from Mouse platelet lysate with ab289964 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289964 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse platelet lysate 10 µg

      Lane 2: ab289964 IP in Mouse platelet lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289964 in mouse platelet lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 15 seconds

      The expression profile/ molecµlar weight observed is consistent with what has been described in the literature (PMID: 23112346, PMID: 9295288).

    • Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
      Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
      Anti-GPVI antibody [EPR25283-14] (ab289964) at 1/1000 dilution + Mouse platelet lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

      Predicted band size: 37 kDa



      This data was developed using 289964, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: The expression profile/ molecular weight  observed is consistent with what has been described in the literature (PMID:�23112346, PMID:�16014566  ).

      114 seconds

      Exposure time: 

    • Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
      Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)

      This data was developed using ab289964, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling GPVI with ab289964 at 1/100 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Negative control (PMID: 10961879). No staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)

      This data was developed using ab289964, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling GPVI with ab289964 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: no staining on mouse cerebrum PMID 10961879). The section was incubated with ab289964 at 4°C overnight. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

      Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

    • Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
      Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab289987 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab289987 は論文での使用が確認できていません。

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