Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-GPR56 抗体 [EPR26546-78] - BSA and Azide free (ab302910)

Submit a review Submit a question
Western blot - Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)
  • Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26546-78] to GPR56 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Unconjugated

製品の概要

  • 製品名

    Anti-GPR56 antibody [EPR26546-78] - BSA and Azide free
    GPR56 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26546-78] to GPR56 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human cerebellum tissue lysate. U-87 MG whole cell lysate. IHC-P: Human astrocytoma tissue. HEK-293T cells transfected with a GPR56 expression vector containing a his tag.

  • 特記事項

    AB302910 is a carrier free version of AB302909. 

    AB302909 does not react in: WB and IHC-P with mouse and rat samples; ICC/IF, Flow Cytometry and IP with human samples. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302910の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 78 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 78 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Could be involved in cell-cell interactions.

  • 組織特異性

    Widely distributed with highest levels found in thyroid gland, brain and heart. Expressed in a great number of tumor cells.

  • 関連疾患

    Polymicrogyria, bilateral frontoparietal (BFPP) [MIM:606854]: A malformation of the cortex in which the brain surface is irregular and characterized by an excessive number of small gyri with abnormal lamination, most severe in the frontoparietal regions. BFPP clinical manifestations include developmental and psychomotor delay, cerebellar and pyramidal signs, truncal ataxia, seizures, hyperreflexia. Polymicrogyria is a heterogeneous disorder, considered to be the result of postmigratory abnormal cortical organization. Note=The disease is caused by mutations affecting the gene represented in this entry.

  • 配列類似性

    Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 1 GPS domain.

  • 翻訳後修飾

    The endogenous protein is proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 7 transmembrane protein with no EGF like N terminal domains 1 antibody
      • BFPP antibody
      • DKFZp781L1398 antibody
      • EGF TM7 like antibody
      • G protein coupled receptor 56 antibody
      • G-protein coupled receptor 56 antibody
      • GPR 56 antibody
      • Gpr56 antibody
      • GPR56_HUMAN antibody
      • Polymicrogyria bilateral frontoparietal antibody
      • Protein TM7XN1 antibody
      • TM7LN4 antibody
      • TM7XN1 antibody
      • TM7XN1 protein antibody
      see all

    画像

    • Western blot - Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)
      Western blot - Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)
      All lanes : Anti-GPR56 antibody [EPR26546-78] (ab302909) at 1/1000 dilution

      Lane 1 : Human cerebellum tissue lysate
      Lane 2 : Human lung tissue lysate
      Lane 3 : Human liver tissue lysate
      Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 78 kDa
      Observed band size: 65 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using AB302909, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer: 5% NFDM/TBST. 

      Negative control: lung, liver (PMID: 15674329). 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)

      This data was developed using AB302909, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling GPR56 with ab302909 at 1/500 dilution (5.38 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human astrocytoma is observed (PMID: 15674329). The section was incubated with ab302909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)

      This data was developed using AB302909, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human liver tissue labeling GPR56 with ab302909 at 1/500 dilution (5.38 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). The section was incubated with ab302909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Negative control: No staining on human liver is observed (PMID: 15674329).

      Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)

      This data was developed using AB302909, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded transfected HEK-293T cells labeling GPR56 with ab302909 at 1/5000 dilution (5.38 µg/ml), followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on (Panel A) HEK-293T cells transfected with a GPR56 expression vector containing a his tag is observed. No staining on (Panel B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab302909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)
      Anti-GPR56 antibody [EPR26546-78] (BSA and Azide free) (AB302910)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302910 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302910 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab302910.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com