Anti-GPNMB 抗体 [7C10E5] (ab175427)
Key features and details
- Mouse monoclonal [7C10E5] to GPNMB
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GPNMB antibody [7C10E5]
GPNMB 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7C10E5] to GPNMB -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human GPNMB aa 31-260.
Sequence:NERPSAYMREHNQLNGWSSDENDWNEKLYPVWKRGDMRWKNSWKGGRVQA VLTSDSPALVGSNITFAVNLIFPRCQKEDANGNIVYEKNCRNEAGLSADP YVYNWTAWSEDSDGENGTGQSHHNVFPDGKPFPHHPGWRRWNFIYVFHTL GQYFQKLGRCSVRVSVNTANVTLGPQLMEVTVYRRHGRAYVPIAQVKDVY VVTDQIPVFVTMFQKNDRNSSDETFLKDLP
Database link: Q14956 -
ポジティブ・コントロール
- GPNMB recombinant protein; GPNMB-hIgGFc transfected HEK293 cell lysate; PANC1 and PC-3 cell lysate; Breast cancer tissues; Esophagus cancer tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
7C10E5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175427の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 64 kDa.
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IHC-P | (1) |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 64 kDa. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Could be a melanogenic enzyme. -
組織特異性
Not restricted to the melanocytic lineage. -
配列類似性
Belongs to the PMEL/NMB family.
Contains 1 PKD domain. -
発生段階
Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines. -
細胞内局在
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 10457 Human
- Omim: 604368 Human
- SwissProt: Q14956 Human
- Unigene: 190495 Human
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別名
- Glycoprotein (transmembrane) nmb antibody
- Glycoprotein nmb antibody
- Glycoprotein nmb like protein antibody
see all
画像
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All lanes : Anti-GPNMB antibody [7C10E5] (ab175427) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : GPNMB (AA: 31-260)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 64 kDa -
Anti-GPNMB antibody [7C10E5] (ab175427) at 1/500 dilution + GPNMB recombinant protein.
Predicted band size: 64 kDa -
All lanes : Anti-GPNMB antibody [7C10E5] (ab175427) at 1/500 dilution
Lane 1 : PANC1 cell lysate.
Lane 2 : PC-3 cell lysate
Predicted band size: 64 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [7C10E5] (ab175427)
Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues labeling GPNMB wiith ab175427 at 1/200 diution, with DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [7C10E5] (ab175427)
Immunohistochemical analysis of paraffin-embedded breast cancer tissues labeling GPNMB wiith ab175427 at 1/200 diution, with DAB staining.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab175427 は論文での使用が確認できていません。