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Anti-GPNMB 抗体 [7C10E5] (ab175427)

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Western blot - Anti-GPNMB antibody [7C10E5] (ab175427)
  • Western blot - Anti-GPNMB antibody [7C10E5] (ab175427)
  • Western blot - Anti-GPNMB antibody [7C10E5] (ab175427)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [7C10E5] (ab175427)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [7C10E5] (ab175427)

Key features and details

  • Mouse monoclonal [7C10E5] to GPNMB
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-GPNMB antibody [SP299] (ab227695)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-GPNMB antibody [7C10E5]
    GPNMB 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [7C10E5] to GPNMB

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human, Recombinant fragment

  • 免疫原

    Recombinant fragment corresponding to Human GPNMB aa 31-260.
    Sequence:

    NERPSAYMREHNQLNGWSSDENDWNEKLYPVWKRGDMRWKNSWKGGRVQA VLTSDSPALVGSNITFAVNLIFPRCQKEDANGNIVYEKNCRNEAGLSADP YVYNWTAWSEDSDGENGTGQSHHNVFPDGKPFPHHPGWRRWNFIYVFHTL GQYFQKLGRCSVRVSVNTANVTLGPQLMEVTVYRRHGRAYVPIAQVKDVY VVTDQIPVFVTMFQKNDRNSSDETFLKDLP


    Database link: Q14956
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • GPNMB recombinant protein; GPNMB-hIgGFc transfected HEK293 cell lysate; PANC1 and PC-3 cell lysate; Breast cancer tissues; Esophagus cancer tissues.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 特記事項（精製）

    Purified from tissue culture supernatant.

  • ポリ/モノ

    モノクローナル

  • クローン名

    7C10E5

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab175427の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/2000. Predicted molecular weight: 64 kDa.
IHC-P (1)
Use at an assay dependent concentration.
特記事項
WB
1/500 - 1/2000. Predicted molecular weight: 64 kDa.
IHC-P
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Could be a melanogenic enzyme.

  • 組織特異性

    Not restricted to the melanocytic lineage.

  • 配列類似性

    Belongs to the PMEL/NMB family.
    Contains 1 PKD domain.

  • 発生段階

    Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines.

  • 細胞内局在

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Glycoprotein (transmembrane) nmb antibody
      • Glycoprotein nmb antibody
      • Glycoprotein nmb like protein antibody
      • Gpnmb antibody
      • GPNMB_HUMAN antibody
      • HGFIN antibody
      • NMB antibody
      • Osteoactivin antibody
      • Transmembrane glycoprotein antibody
      • Transmembrane glycoprotein HGFIN antibody
      • Transmembrane glycoprotein NMB antibody
      see all

    画像

    • Western blot - Anti-GPNMB antibody [7C10E5] (ab175427)
      Western blot - Anti-GPNMB antibody [7C10E5] (ab175427)
      All lanes : Anti-GPNMB antibody [7C10E5] (ab175427) at 1/500 dilution

      Lane 1 : HEK293 cell lysate
      Lane 2 : GPNMB (AA: 31-260)-hIgGFc transfected HEK293 cell lysate

      Predicted band size: 64 kDa

    • Western blot - Anti-GPNMB antibody [7C10E5] (ab175427)
      Western blot - Anti-GPNMB antibody [7C10E5] (ab175427)
      Anti-GPNMB antibody [7C10E5] (ab175427) at 1/500 dilution + GPNMB recombinant protein.

      Predicted band size: 64 kDa

    • Western blot - Anti-GPNMB antibody [7C10E5] (ab175427)
      Western blot - Anti-GPNMB antibody [7C10E5] (ab175427)
      All lanes : Anti-GPNMB antibody [7C10E5] (ab175427) at 1/500 dilution

      Lane 1 : PANC1 cell lysate.
      Lane 2 : PC-3 cell lysate

      Predicted band size: 64 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [7C10E5] (ab175427)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [7C10E5] (ab175427)

      Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues labeling GPNMB wiith ab175427 at 1/200 diution, with DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [7C10E5] (ab175427)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [7C10E5] (ab175427)

      Immunohistochemical analysis of paraffin-embedded breast cancer tissues labeling GPNMB wiith ab175427 at 1/200 diution, with DAB staining.

    参考文献 (0)

    ab175427 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab175427 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GPNMB antibody [7C10E5]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA
    Permeabilization
    Yes - 3% Hydrogen Peroxide
    Specification
    Lung
    Blocking step
    Background Blask as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Chenghao Zhang

    Verified customer

    投稿 Dec 07 2021

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