AB138509

Anti-GPCR RDC1/CXCR-7 抗体 [EPR9321]

Name: Anti-GPCR RDC1/CXCR-7 抗体 [EPR9321] (ab138509) | Abcam
Brand: Abcam Limited
SKU: AB138509
Price: 33000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-GPCR RDC1/CXCR-7 antibody [EPR9321]

20ul selling size
RabMAb
Recombinant
詳細を見る

(1 Review)

(13 Publications)

Rabbit Recombinant Monoclonal GPCR RDC1/CXCR-7 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 13 publications.

別名を表示する

CMKOR1, CXCR7, GPR159, RDC1, ACKR3, Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, Chemokine orphan receptor 1, G-protein coupled receptor 159, G-protein coupled receptor RDC1 homolog, CXC-R7, CXCR-7, RDC-1

3 Images

Unknown

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (AB138509)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (ab138509) at 1/10000 dilution

All lanes:

THP-1 (Human monocytic leukemia monocyte) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 41 kDa

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Unknown

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (AB138509)

All lanes:

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (ab138509) at 1/1000 dilution

Lane 1:

THP-1 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Lane 5:

Raw 264.7 cell lysate at 10 µg

Lane 6:

NIH3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Unknown

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (AB138509)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (ab138509) at 1/10000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

Lane 2:

C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 41 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR9321

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GPCR RDC1 also known as CXCR-7 is a G protein-coupled receptor that binds to chemokines such as CXCL12 and CXCL11. The receptor has a predicted molecular mass of approximately 40 kDa. It expresses widely in various tissues with high levels found in the heart liver kidney and brain. This receptor plays a role in the trafficking and localization of different cells often working alongside or complementing other chemokine receptors.

Biological function summary

GPCR RDC1/CXCR-7 takes part in complex cell signaling related to cell growth survival and migration. It is not usually part of a classic GPCR signaling pathway which sets it apart from other receptors in its family. Instead it can modulate pathways by forming heterodimers with other receptors such as CXCR4 affecting both ligand availability and receptor sensitivity. CXCR-7 can also function as a decoy receptor binding ligands without initiating a typical signaling cascade.

Pathways

The participation of GPCR RDC1/CXCR-7 is noted in chemokine-mediated pathways. Of particular importance is the SDF-1/CXCL12 signaling pathway which it shares with CXCR4. This pathway plays an important role in the regulation of stem cell migration and homing. CXCR-7 modulates the bioavailability of CXCL12 and influences how CXCR4 triggers downstream effects affecting cellular response to the external environment.

GPCR RDC1/CXCR-7 holds significance in cancer and cardiovascular diseases. In cancer overexpression of CXCR-7 associates with tumor growth and metastasis often in conjunction with the action of CXCR4. In cardiovascular conditions altered expression and functional shifts in CXCR-7 can lead to abnormal vascularization contributing to disease progression. Its role in these diseases makes CXCR-7 a valuable target for therapeutic interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1 (PubMed : 16107333, PubMed : 19255243, PubMed : 19380869, PubMed : 20161793, PubMed : 22300987). Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway (PubMed : 16940167, PubMed : 18653785, PubMed : 20018651). Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness (PubMed : 16940167, PubMed : 18653785). In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival (PubMed : 16940167, PubMed : 20388803). Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration (PubMed : 17804806, PubMed : 18653785, PubMed : 19641136, PubMed : 20887389). Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12 (PubMed : 18653785). Required for heart valve development (PubMed : 17804806). Regulates axon guidance in the oculomotor system through the regulation of CXCL12 levels (PubMed : 31211835).. (Microbial infection) Acts as a coreceptor with CXCR4 for a restricted number of HIV isolates.

See full target information ACKR3

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38994928

2024

Roxadustat Attenuates Adverse Remodeling Following Myocardial Infarction in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Marc-Michael Zaruba,Simon Staggl,Santhosh Kumar Ghadge,Thomas Maurer,Jasmina Gavranovic-Novakovic,Vivek Jeyakumar,Patric Schönherr,Andreas Wimmer,Gerhard Pölzl,Axel Bauer,Moritz Messner

Translational pediatrics 12:1634-1645 PubMed37814711

2023

CXCR7 promotes the migration of fibroblasts derived from patients with acquired laryngotracheal stenosis by NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Mengrou Xu,Bin Hu,Jiarui Chen,Limin Zhao,Jing Wang,Xiaoyan Li

Bioengineered 13:7425-7438 PubMed35264069

2022

C-X-C motif chemokine ligand 12 (CXCL12)/C-X-C motif chemokine receptor 7(CXCR7) regulates epithelial-mesenchymal transition process and promotes the metastasis of esophageal cancer by activating signal transducer and activator of transcription 3 (STAT3) pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Guo,Chang-Yong Tong,Jian-Guang Shi,Xin-Jian Li

Frontiers in molecular biosciences 8:652443 PubMed34746227

2021

Enzalutamide-Resistant Progression of Castration-Resistant Prostate Cancer Is Driven via the JAK2/STAT1-Dependent Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Luo,Xiaobing Yang,Spyridon P Basourakos,Xuemei Zuo,Dechao Wei,Jiahui Zhao,Mingchuan Li,Qiankun Li,Tao Feng,Pengju Guo,Yongguang Jiang

Stem cell research & therapy 12:264 PubMed33941256

2021

Upregulating CXCR7 accelerates endothelial progenitor cell-mediated endothelial repair by activating Akt/Keap-1/Nrf2 signaling in diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyu Jiang,Ruiting Li,Chaoyang Xiu,Xu Ma,Hui Hu,Liming Wei,Yihan Tang,Mingyang Tao,Jungong Zhao

Marine drugs 19: PubMed33805447

2021

Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Hung Guo,Simon Hsia,Chieh-Han Chung,Yi-Chun Lin,Min-Yi Shih,Pei-Chung Chen,Guoo-Shyng W Hsu,Ciou-Ting Fan,Chia-Lin Peng

Respiratory research 21:287 PubMed33129326

2020

Overexpression of CXCR7 accelerates tumor growth and metastasis of lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Liu,Qian Cheng,Dong-Sheng Xu,Wen Wang,Zheng Fang,Dong-Dong Xue,Ya Zheng,Alex H Chang,Yan-Jun Lei

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 69:167-178 PubMed31865399

2019

Modulation of CXC-motif chemokine receptor 7, but not 4, expression is related to migration of the human prostate cancer cell LNCaP: regulation by androgen and inflammatory stimuli.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Yu,Quynhchi Pham,Liangli Lucy Yu,Thomas T Y Wang

Experimental and therapeutic medicine 18:1091-1098 PubMed31316604

2019

Forkhead box P3 gene silencing inhibits the expression of chemokines and chemokine receptors associated with cell growth, migration, and apoptosis in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Ou,Guang-Tao Zhang,Pei-Kai Tian,Jing-Sen Chen,Ze-Wei Lin,Yong Xie,Ai-Hong Wang,Xiao-Ping Liu,Ji-Kui Liu

Experimental cell research 364:175-183 PubMed29408206

2018

The 5T4 oncofetal glycoprotein does not act as a general organizer of the CXCL12 system in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Malte Puchert,Christian Koch,Jürgen Engele

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPCR RDC1/CXCR-7 抗体 [EPR9321]

Anti-GPCR RDC1/CXCR-7 antibody [EPR9321]

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (AB138509)

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (AB138509)

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (AB138509)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (13)

代替製品

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Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise