Anti-GPCR GPR87 抗体 (ab77517)
Key features and details
- Rabbit polyclonal to GPCR GPR87
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GPCR GPR87 antibody
GPCR GPR87 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GPCR GPR87 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide derived from internal sequence of human GPCR GPR87.
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ポジティブ･コントロール
- Extracts from HUVEC cells and HT-29 cells. HUVEC cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77517の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/1000. Predicted molecular weight: 41 kDa.
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| ICC/IF |
1/500 - 1/1000.
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Predicted molecular weight: 41 kDa. |
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Receptor for lysophosphatidic acid (LPA). Necessary for p53/TP53-dependent survival in response to DNA damage. -
組織特異性
Expressed in placenta and prostate. Weaker expression in thymus. Not expressed in thalamus, hippocampus, pons or cerebellum. Overexpressed in squamous cell carcinoma of the lung. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 53836 Human
- Entrez Gene: 84111 Mouse
- Omim: 606379 Human
- SwissProt: Q9BY21 Human
- SwissProt: Q99MT7 Mouse
- Unigene: 591292 Human
- Unigene: 60267 Mouse
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別名
- FKSG 78 antibody
- FKSG78 antibody
- G protein coupled receptor 87 antibody
see all
画像
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Western blot - Anti-GPCR GPR87 antibody (ab77517)All lanes : Anti-GPCR GPR87 antibody (ab77517) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from HT-29 cells
Lane 3 : Extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 41 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 25 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR87 antibody (ab77517)Immunofluorescence analysis of GPCR GPR87 in HUVEC cells using ab77517, at 1/500 dilution, in the absence or presence of immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab77517 は 4 報の論文で使用されています。
- Jiang J et al. G Protein-Coupled Receptor GPR87 Promotes the Expansion of PDA Stem Cells through Activating JAK2/STAT3. Mol Ther Oncolytics 17:384-393 (2020). PubMed: 32405536
- Yu M et al. Taurine attenuates gossypol-induced apoptosis of C2C12 mouse myoblasts via the GPR87-AMPK/AKT signaling. Amino Acids 52:1285-1298 (2020). PubMed: 32918616
- Yu M et al. Taurine Promotes Milk Synthesis via the GPR87-PI3K-SETD1A Signaling in BMECs. J Agric Food Chem 67:1927-1936 (2019). PubMed: 30678459
- Zhang X et al. G Protein-Coupled Receptor 87 (GPR87) Promotes Cell Proliferation in Human Bladder Cancer Cells. Int J Mol Sci 16:24319-31 (2015). WB . PubMed: 26473854