Anti-GPCR GPR35 抗体 (ab76217)
Key features and details
- Rabbit polyclonal to GPCR GPR35
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GPCR GPR35 antibody
GPCR GPR35 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GPCR GPR35 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: extracts from LOVO cells; ICC/IF: A549 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76217の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 34 kDa.
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| ICC/IF |
1/500 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 34 kDa. |
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of this receptor is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins. -
組織特異性
Predominantly expressed in immune and gastrointestinal tissues. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. Internalized to the cytoplasm after exposure to kynurenic acid. - Information by UniProt
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参照データベース
- Entrez Gene: 2859 Human
- Omim: 602646 Human
- SwissProt: Q9HC97 Human
- Unigene: 112218 Human
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別名
- G protein coupled receptor 35 antibody
- G protein coupled receptor 35 antibody
- G-protein coupled receptor 35 antibody
see all
画像
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Western blot - Anti-GPCR GPR35 antibody (ab76217)All lanes : Anti-GPCR GPR35 antibody (ab76217) at 1/500 dilution
Lane 1 : extracts from LOVO cells
Lane 2 : extracts from LOVO cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR35 antibody (ab76217)Left panel: immunofluorescence analysis of A549 cells, using 1/500 ab76217. Right panel: same, after pre-incubation with the immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab76217 は 2 報の論文で使用されています。
- Bu J et al. Activation of the IL-17 signalling pathway by the CXCL17-GPR35 axis affects drug resistance and colorectal cancer tumorigenesis. Am J Cancer Res 13:2172-2187 (2023). PubMed: 37293165
- Wang W et al. Overexpression of GPR35 confers drug resistance in NSCLC cells by ß-arrestin/Akt signaling. Onco Targets Ther 11:6249-6257 (2018). PubMed: 30288060