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Anti-GPCR GPR126 抗体 (ab75356)

Submit a review Q&A (3)References (5)
Western blot - Anti-GPCR GPR126 antibody (ab75356)

    Key features and details

    • Rabbit polyclonal to GPCR GPR126
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-GPCR GPR126 antibody
      GPCR GPR126 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to GPCR GPR126

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide derived from an internal region of human GPCR GPR126

    • ポジティブ･コントロール

      • Extracts from HUVEC cells

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • バッファー

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

      Without Mg2+ and Ca2+
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab75356の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/500 - 1/1000. Detects a band of approximately 137 kDa (predicted molecular weight: 137 kDa).
    特記事項
    WB
    1/500 - 1/1000. Detects a band of approximately 137 kDa (predicted molecular weight: 137 kDa).

    ターゲット情報

    • 機能

      Orphan receptor. May be required for normal differentiation of promyelinating Schwann cells and for normal myelination of axons. Signals probably through G-proteins to transiently elevate cAMP levels.

    • 組織特異性

      Expressed in placenta and to a lower extent in pancreas and liver. Detected in aortic endothelial cells but not in skin microvascular endothelial cells.

    • 配列類似性

      Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
      Contains 1 CUB domain.
      Contains 1 GPS domain.
      Contains 1 pentaxin domain.

    • 翻訳後修飾

      Proteolytically cleaved into 2 conserved sites: one in the GPS domain (S1 site) and the other in the middle of the extracellular domain (S2 site). The proteolytic cleavage at S1 site generates an extracellular subunit and a seven-transmembrane subunit. Furin is involved in the cleavage of the S2 site generating a soluble fragment. Processing at the GPS domain occurred independent of and probably prior to the cleavage at the S2 site.

    • 細胞内局在

      Cell membrane. Detected on the cell surface of activated but not resting umbilical vein.
    • Information by UniProt

    • 参照データベース

      • 別名

        • adhesion G protein coupled receptor G6 antibody
        • APG1 antibody
        • Developmentally regulated G protein coupled receptor antibody
        • Developmentally regulated G-protein-coupled receptor antibody
        • dj287g14.2 antibody
        • DREG antibody
        • G protein coupled receptor 126 antibody
        • G-protein coupled receptor 126 antibody
        • GP126_HUMAN antibody
        • GPR126 antibody
        • HBV PreS1 transactivated protein 2 antibody
        • HBV PreS1-transactivated protein 2 antibody
        • LCCS9 antibody
        • OTTHUMP00000017320 antibody
        • OTTHUMP00000237093 antibody
        • OTTHUMP00000237094 antibody
        • OTTHUMP00000237115 antibody
        • PR126 antibody
        • Probable G protein coupled receptor 126 [Precursor] antibody
        • Probable G protein coupled receptor 126 antibody
        • Probable G-protein coupled receptor 126 [Precursor] antibody
        • Probable G-protein coupled receptor 126 antibody
        • PS1TP2 antibody
        • Vascular inducible G protein coupled receptor antibody
        • Vascular inducible G protein-coupled receptor antibody
        • VIGR antibody
        see all

      画像

      • Western blot - Anti-GPCR GPR126 antibody (ab75356)
        Western blot - Anti-GPCR GPR126 antibody (ab75356)
        All lanes : Anti-GPCR GPR126 antibody (ab75356) at 1/500 dilution

        Lane 1 : extracts from HUVEC cells
        Lane 2 : extracts from HUVEC cells with immunising peptide at 10 µg

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 137 kDa
        Observed band size: 137 kDa

      参考文献 (5)

      ab75356 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab75356 は 5 報の論文で使用されています。

      • Cui H  et al. GPR126 regulates colorectal cancer cell proliferation by mediating HDAC2 and GLI2 expression. Cancer Sci 112:1798-1810 (2021). PubMed: 33629464
      • Wu S  et al. Whole-genome sequencing identifies ADGRG6 enhancer mutations and FRS2 duplications as angiogenesis-related drivers in bladder cancer. Nat Commun 10:720 (2019). PubMed: 30755618
      • Xu E  et al. Asymmetric expression of GPR126 in the convex/concave side of the spine is associated with spinal skeletal malformation in adolescent idiopathic scoliosis population. Eur Spine J 28:1977-1986 (2019). PubMed: 31079250
      • Kitagaki J  et al. A Putative Association of a Single Nucleotide Polymorphism in GPR126 with Aggressive Periodontitis in a Japanese Population. PLoS One 11:e0160765 (2016). WB . PubMed: 27509131
      • Cui H  et al. GPR126 protein regulates developmental and pathological angiogenesis through modulation of VEGFR2 receptor signaling. J Biol Chem 289:34871-85 (2014). PubMed: 25217645

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-3 of 3 Abreviews or Q&A

      Question

      Has your Anti-GPCR GPR126 antibody (ab75356) been tested in mouse for IHC? Is it predicted to cross-react with mouse?

      Read More
      Answer


      The antibody has not been predicted to cross react with mouse GPR126. The lab aligned the exact immunogenic sequence used to raise the antibody with Q6F3F9, and the homology is only 64.3%.

      We usually say that a homology of 85% or higher has a good likelihood for crossreactivity.

      Read More

      Question

      Thank-you for your help. I'll let you know if we decide to get any of the antibodies, although at first glance there does not appear to be good conservation to these peptides.

      Read More
      Answer

      Thank you for this feedback. I am sorry that I could not be of more help on this occasion. If you like to get some more information on our testing discount program, please find the Terms and Conditions of this offer at: www.abcam.com/collaborationdiscount. We wish you the best of luck with your research. Should you have any further questions, please do not hesitate to contact us.

      Read More

      Question

      I am trying to find an antibody against gpr126 that will cross react with zebrafish. Please can you tell me the peptide sequence that was used to raise antibodies ab65175, ab75356 and ab117092, so that I can see if they are from a conserved region of the protein. Best wishes Sarah

      Read More
      Answer

      Thank you for contacting us. I am happy to let you know the following immunogen sequences: ab65175: xxxxxxxxxxxxxxxxxx ab75356: yyyyyyyyyyyyyyyy In preliminary searches, I could not find conclusive matches for these peptides in zebrafish (only 75-80% similarity with various proteins); however, I trust that you can determine whether the antibodies would potentially cross-react. The sequence of the immunogenic peptide used to generate antibody ab117092 is considered proprietary. An alignment with Zebrafish revealed only 65% homology between the sequence of the human peptide used as the immunogen and the corresponding region in Zebrafish GPR126. Based on these similarity figures we would not expect these antibodies to perform well with Zebrafish samples. Having said this, we realise how difficult it is to find suitable antibodies for Zebrafish. If you therefore nevertheless decide to go ahead and purchase one of these products, please contact us again prior to the purchase as you might be eligible for our testing discount program. Please find also attached a list of our antibodies that are tested and guaranteed to work with Zebrafish for your information. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

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