Anti-GPCR GPR119 抗体 (ab75312)
Key features and details
- Rabbit polyclonal to GPCR GPR119
- Suitable for: ELISA, IHC-Fr, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GPCR GPR119 antibody
GPCR GPR119 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GPCR GPR119 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IHC-Fr, IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide:
KMEHAGAMAGGYRSPRTPSDFKALRTVSVL
, corresponding to internal sequence amino acids 201-230 of Human GPCR GPR119 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75312の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| WB |
1/500 - 1/1000. Predicted molecular weight: 37 kDa.
|
|
| ICC/IF |
1/500 - 1/1000.
|
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/500 - 1/1000. Predicted molecular weight: 37 kDa. |
|
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Receptor for the endogenous fatty-acid ethanolamide oleoylethanolamide (OEA) and lysophosphatidylcholine (LPC). Functions as a glucose-dependent insulinotropic receptor. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Seems to act through a G(s) mediated pathway. -
組織特異性
Predominantly expressed in the pancreas, especially in the islets. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 139760 Human
- Entrez Gene: 236781 Mouse
- Omim: 300513 Human
- SwissProt: Q8TDV5 Human
- SwissProt: Q7TQP3 Mouse
- Unigene: 496762 Human
- Unigene: 269129 Mouse
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別名
- G protein coupled receptor 119 antibody
- G protein coupled receptor 2 antibody
- G-protein coupled receptor 119 antibody
see all
画像
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Western blot - Anti-GPCR GPR119 antibody (ab75312)All lanes : Anti-GPCR GPR119 antibody (ab75312) at 1/500 dilution
Lane 1 : extracts from K562 cells
Lane 2 : extracts from K562 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Additional bands at: 28 kDa, 60 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR119 antibody (ab75312)Left panel: immunofluorescence analysis of MCF-7 cells, using 1/500 ab75312. Right panel: same, after pre-incubation with the immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab75312 は 2 報の論文で使用されています。
- Markovics A et al. GPR119 Is a Potent Regulator of Human Sebocyte Biology. J Invest Dermatol 140:1909-1918.e8 (2020). PubMed: 32142797
- Little TJ et al. Characterization of duodenal expression and localization of fatty acid-sensing receptors in humans: relationships with body mass index. Am J Physiol Gastrointest Liver Physiol 307:G958-67 (2014). PubMed: 25258406