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AB324954

Anti-GPC2 antibody [EPR30350-587]

Anti-GPC2 antibody [EPR30350-587]

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Rabbit Recombinant Monoclonal GPC2 antibody. Suitable for WB, I-ELISA and reacts with Human, Recombinant full length protein - Human samples.

別名を表示する

Glypican-2, GPC2

2 Images
ELISA - Anti-GPC2 antibody [EPR30350-587] (AB324954)
  • ELISA

Supplier Data

ELISA - Anti-GPC2 antibody [EPR30350-587] (AB324954)

Indirect ELISA analysis of ab324954 at 1000-0 ng/ml. The Secondary antibody used was Peroxidase AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific at 1 : 20000 dilution dilution.

Antigen : Human GPC2 protein, Human GPC6 protein, Human GPC1 protein.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with human GPC1 and GPC6.

Western blot - Anti-GPC2 antibody [EPR30350-587] (AB324954)
  • WB

Supplier Data

Western blot - Anti-GPC2 antibody [EPR30350-587] (AB324954)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HeLa, 293T, ARPE-19 (PMID : 34195677).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 34195677; PMID : 34337560; PMID : 12084985).

The identity of the higher MW bands observed in lanes 1–2 are most likely glycosylated forms of GPC2 (PMID : 34337560).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 136 seconds; Lanes 2-5 : 70 seconds.

All lanes:

Western blot - Anti-GPC2 antibody [EPR30350-587] (ab324954) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 48 µg

Lane 2:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 48 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate at 48 µg

Lane 5:

ARPE-19 (human eye epithelial cell) whole cell lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 62 kDa,80-300 kDa,36 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR30350-587

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, I-ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Unsuitable for WB in human tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "15.625 ng/mL", "IELISA-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information GPC2
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

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