Anti-GPAM 抗体 (ab69990)
Key features and details
- Rabbit polyclonal to GPAM
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-GPAM antibody
GPAM 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GPAM -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat -
免疫原
15 amino acid peptide near the carboxy terminus of the human GPAM (GenBank accession no. NP_065969).
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ポジティブ･コントロール
- WB: Rat brain tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69990の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 94 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 94 kDa). |
ターゲット情報
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機能
Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. -
パスウェイ
Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. -
配列類似性
Belongs to the GPAT/DAPAT family. -
ドメイン
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
細胞内局在
Mitochondrion outer membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 29653 Rat
- SwissProt: P97564 Rat
- Unigene: 44456 Rat
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別名
- Glycerol 3 phosphate acyltransferase 1 mitochondrial antibody
- Glycerol 3 phosphate acyltransferase mitochondrial antibody
- Glycerol-3-phosphate acyltransferase 1 antibody
see all
画像
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Western blot - Anti-GPAM antibody (ab69990)Lane 1 : Anti-GPAM antibody (ab69990) at 1 µg/ml
Lane 2 : Anti-GPAM antibody (ab69990) at 2 µg/ml
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 94 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab69990 は 5 報の論文で使用されています。
- Godoy-Lugo JA et al. Chronic angiotensin receptor activation promotes hepatic triacylglycerol accumulation during an acute glucose challenge in obese-insulin-resistant OLETF rats. Endocrine 75:92-107 (2022). PubMed: 34327606
- Wang JH et al. Bacillus subtilis-Fermented Amomum xanthioides Ameliorates Metabolic-Syndrome-Like Pathological Conditions in Long-Term HFHFD-Fed Mice. Antioxidants (Basel) 11:N/A (2022). PubMed: 36421440
- Mendham AE et al. Exercise training improves mitochondrial respiration and is associated with an altered intramuscular phospholipid signature in women with obesity. Diabetologia N/A:N/A (2021). PubMed: 33770195
- Chi Y et al. Long noncoding RNA lncARSR promotes nonalcoholic fatty liver disease and hepatocellular carcinoma by promoting YAP1 and activating the IRS2/AKT pathway. J Transl Med 18:126 (2020). PubMed: 32169080
- Beaudoin MS et al. Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats. Obesity (Silver Spring) 22:1632-42 (2014). PubMed: 24500776