AB314107

Anti-GP130/CD130 抗体 [BLR114H] - BSA free

Anti-GP130/CD130 antibody [BLR114H] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal CD130 (gp130) antibody. Suitable for ICC, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL6ST aa 1-350.

別名を表示する

CD130, Interleukin-6 receptor subunit beta, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, CDw130, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin-M receptor subunit alpha, gp130, IL6ST

4 Images

Supplier Data

Immunocytochemistry - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Detection of human GP130/CD130 in FFPE HeLa cells by immunocytochemistry Antibody : Rabbit antiGP130/CD130 recombinant monoclonal antibody [BLR114H] (A700-114). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunocytochemistry - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Detection of human GP130/CD130 in FFPE GaMG cells by immunocytochemistry. Antibody : Rabbit antiGP130/CD130 recombinant monoclonal antibody [BLR114H] (A700-114). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunocytochemistry - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Detection of human GP130/CD130 in FFPE HUVEC-C cells by immunocytochemistry. Antibody : Rabbit antiGP130/CD130 recombinant monoclonal antibody [BLR114H] (A700-114). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Western blot - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Samples : Whole cell lysate (50 µg) from NCI-H226, MCF-7, IMR-90, Jurkat, HeLa, A-549, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody : Rabbit antiGP130/CD130 recombinant monoclonal antibody [BLR114H] (A700-114) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit anti-COPB2.

All lanes:

Western blot - Anti-GP130/CD130 antibody [BLR114H] - BSA free (ab314107) at 1/1000 dilution

Lane 1:

NCI-H226 Whole cell lysate at 50 µg

Lane 2:

MCF-7 Whole cell lysate at 50 µg

Lane 3:

IMR-90 Whole cell lysate at 50 µg

Lane 4:

Jurkat, Whole cell lysate at 50 µg

Lane 5:

HeLa Whole cell lysate at 50 µg

Lane 6:

A-549 Whole cell lysate at 50 µg

Lane 7:

Hep-G2 Whole cell lysate at 50 µg

Lane 8:

HEK293T Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

BLR114H

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, ICC

applications

免疫原

Recombinant Fragment Protein within Human IL6ST aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P40189

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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製品の詳細

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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出荷温度及び保存条件

製品の状態

Liquid

バッファー組成

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

出荷温度

Blue Ice

短期保存期間

Up to 12 months

短期保存温度

+4°C

長期保存温度

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD130 also known as gp130 or the IL-6 signal transducer (IL6ST) is a protein weighing around 130 kDa. This receptor is encoded by the IL6ST gene and widely expressed on the surface of cells across multiple tissues. CD130 functions mechanically as a signal transducing receptor facilitating the assembly of receptor complexes necessary for the initiation of signal transduction. It plays a major role in cytokine receptor signaling particularly interacting with interleukin-6 (IL-6) family cytokines.

Biological function summary

CD130 functions as part of the high-affinity receptor complex for certain cytokines. It partners with other receptors such as CD126 to form a complex that initiates signaling pathways after ligand binding contributing to a range of immune responses. Its role is thorough across many cellular processes including differentiation proliferation and apoptosis. In addition to immune signaling CD130 is involved in several growth factor signaling pathways.

Pathways

CD130 serves a significant role in signal transduction particularly in the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway. It works in concert with related proteins like JAK1 and STAT3 to transmit signals from extracellular cytokines to the nucleus influencing gene expression. Additionally CD130 can also be found active in the MAPK/ERK pathway which is important for cellular response to growth signals.

CD130 plays a connection especially to inflammatory conditions and certain cancers. Aberrant signaling involving CD130 has been linked to disorders like rheumatoid arthritis where it contributes to the inflammation process. Additionally it relates to multiple myeloma a cancer where abnormal CD130 interactions with proteins like IL-6 may enhance cell survival and proliferation presenting potential targets for therapeutic intervention.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Signal-transducing molecule (PubMed : 2261637). The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates the intracellular JAK-MAPK and JAK-STAT3 signaling pathways (PubMed : 19915009, PubMed : 2261637, PubMed : 23294003). That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3 (PubMed : 19915009, PubMed : 23294003, PubMed : 25731159). In parallel, the IL6 signaling pathway induces the expression of two cytokine receptor signaling inhibitors, SOCS1 and SOCS3, which inhibit JAK and terminate the activity of the IL6 signaling pathway as a negative feedback loop (By similarity). Also activates the yes-associated protein 1 (YAP) and NOTCH pathways to control inflammation-induced epithelial regeneration, independently of STAT3 (By similarity). Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL27RA/WSX1 and CNTFR (PubMed : 19386761). Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism (By similarity). Has a role in embryonic development (By similarity). Essential for survival of motor and sensory neurons and for differentiation of astrocytes (By similarity). Required for expression of TRPA1 in nociceptive neurons (By similarity). Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation (By similarity). Required for normal trabecular bone mass and cortical bone composition (By similarity).. Isoform 2. Binds to the soluble IL6 : sIL6R complex (hyper-IL6), thereby blocking IL6 trans-signaling. Inhibits sIL6R-dependent acute phase response (PubMed : 11121117, PubMed : 21990364, PubMed : 30279168). Also blocks IL11 cluster signaling through IL11R (PubMed : 30279168).

See full target information IL6ST

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GP130/CD130 抗体 [BLR114H] - BSA free

Anti-GP130/CD130 antibody [BLR114H] - BSA free

Immunocytochemistry - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Immunocytochemistry - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Immunocytochemistry - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Western blot - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise