Anti-GORASP2/GRASP55 抗体 (ab68713)
Key features and details
- Mouse polyclonal to GORASP2/GRASP55
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GORASP2/GRASP55 antibody
GORASP2/GRASP55 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to GORASP2/GRASP55 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human GORASP2/GRASP55. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_056345.3, 1 a.a. ~ 452 a.a)
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ポジティブ･コントロール
- mouse neural stem cell lysate, GORASP2/GRASP55 transfected 293T lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68713の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa). |
ターゲット情報
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機能
May be involved in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA. -
配列類似性
Belongs to the GORASP family.
Contains 1 PDZ (DHR) domain. -
翻訳後修飾
Myristoylated. Myristoylation is essential for the Golgi targeting.
Palmitoylated.
Phosphorylated in mitotic cells. -
細胞内局在
Golgi apparatus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 26003 Human
- Omim: 608693 Human
- SwissProt: Q9H8Y8 Human
- Unigene: 431317 Human
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別名
- AW552058 antibody
- DKFZp434D156 antibody
- FLJ13139 antibody
see all
画像
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Western blot - Anti-GORASP2/GRASP55 antibody (ab68713)All lanes : Anti-GORASP2/GRASP55 antibody (ab68713) at 1/500 dilution
Lane 1 : GORASP2 / GRASP5 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 48 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
Western blot - Anti-GORASP2/GRASP55 antibody (ab68713)This image is courtesy of an abreview submitted by Melina Reisenberg, King's College London, United KingdomAnti-GORASP2/GRASP55 antibody (ab68713) at 1/500 dilution + neural stem cell lysate at 30 µg
Secondary
Peroxidase labeled horse anti-mouse IgG (H+L) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
データシートおよび資料
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Datasheet download
参考文献 (1)
ab68713 は 1 報の論文で使用されています。
- Kim YH et al. Secretory autophagy machinery and vesicular trafficking are involved in HMGB1 secretion. Autophagy 17:2345-2362 (2021). PubMed: 33017561
