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AB68713

Anti-GORASP2/GRASP55 抗体

Anti-GORASP2/GRASP55 antibody

4

(1 Review)

|

(1 Publication)

Mouse Polyclonal GORASP2/GRASP55 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GORASP2.

別名を表示する

GOLPH6, GORASP2, Golgi reassembly-stacking protein 2, GRS2, Golgi phosphoprotein 6, Golgi reassembly-stacking protein of 55 kDa, p59, GRASP55

2 Images
Western blot - Anti-GORASP2/GRASP55 antibody (AB68713)
  • WB

AbReview17413****

Western blot - Anti-GORASP2/GRASP55 antibody (AB68713)

All lanes:

Western blot - Anti-GORASP2/GRASP55 antibody (ab68713) at 1/500 dilution

All lanes:

neural stem cell lysate at 30 µg

Secondary

All lanes:

Peroxidase labeled horse anti-mouse IgG (H+L) at 1/3000 dilution

Predicted band size: 47 kDa

Observed band size: 55 kDa

true

Exposure time: 10min

This image is courtesy of an abreview submitted by Melina Reisenberg, King's College London, United Kingdom

Western blot - Anti-GORASP2/GRASP55 antibody (AB68713)
  • WB

Unknown

Western blot - Anti-GORASP2/GRASP55 antibody (AB68713)

All lanes:

Western blot - Anti-GORASP2/GRASP55 antibody (ab68713) at 1/500 dilution

Lane 1:

GORASP2 / GRASP5 transfected 293T lysate at 25 µg

Lane 2:

Non-transfected 293T lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 47 kDa

Observed band size: 52 kDa

false

Key facts

宿主種

Mouse

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human GORASP2.

Q9H8Y8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GORASP2 also known as GRASP55 is a golgi reassembly stacking protein with a mass approximately 55 kDa. It is highly expressed in the Golgi apparatus where it plays an important role in maintaining the structure and function of this organelle. GRASP55 facilitates the stacking of Golgi cisternae which is essential for Golgi apparatus organization. The protein acts alongside its isoform GRASP65 though they function somewhat independently in different Golgi regions.
Biological function summary

GRASP55 controls various cellular processes including the trafficking of proteins and lipids within the cell. It is part of the GRASP complex along with GRASP65 facilitating membrane tethering and fusion events. The protein's function is also linked to its ability to regulate Golgi's response to cellular stress or changes in environment impacting secretion and growth factor availability. In some conditions GRASP55 can influence processes such as autophagy underlining its diverse biological actions within the cell.

Pathways

GRASP55 integrates into significant cellular pathways like the secretory pathway and the autophagy pathway. It interacts with key proteins like golgins and Rab GTPases to assist in vesicle tethering and transport. GRASP55 also closely interacts with p62 a protein that plays a critical role in the selective autophagy pathway. This positions GRASP55 as a contributor to not only the routine processing of cellular materials but also cellular adaptation to stress through autophagy modulation.

GRASP55 shows relevance to cancer progression and neurodegenerative diseases. Aberrant expression or dysfunction of GRASP55 can contribute to cancerous transformations by altering cell adhesion and signaling pathways. Its role in autophagy connects it to diseases like Alzheimer's where defective protein processing is a hallmark. The interaction of GRASP55 with proteins such as p62 becomes particularly important as disrupted autophagy pathways are often implicated in neurodegenerative conditions highlighting GRASP55's significant role in both health and disease.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Key structural protein of the Golgi apparatus (PubMed : 33301566). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (PubMed : 33301566). Acting in concert with GORASP1/GRASP65, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (PubMed : 33301566). However, other studies suggest that GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after breakdown during mitosis and meiosis (PubMed : 10487747, PubMed : 21515684, PubMed : 22523075). May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA (PubMed : 11101516). Required for normal acrosome formation during spermiogenesis and normal male fertility, probably by promoting colocalization of JAM2 and JAM3 at contact sites between germ cells and Sertoli cells (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (PubMed : 21884936, PubMed : 27062250, PubMed : 28067262).
See full target information GORASP2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 17:2345-2362 PubMed33017561

2020

Secretory autophagy machinery and vesicular trafficking are involved in HMGB1 secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Young Hun Kim,Man Sup Kwak,Bin Lee,Jae Min Shin,Sowon Aum,In Ho Park,Min Goo Lee,Jeon-Soo Shin
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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