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Anti-GOLPH3/MIDAS 抗体 (ab69171)

Reviews (5)Q&A (1)References (4)
Western blot - Anti-GOLPH3/MIDAS antibody (ab69171)

    Key features and details

    • Mouse polyclonal to GOLPH3/MIDAS
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-GOLPH3/MIDAS antibody
      GOLPH3/MIDAS 一次抗体 製品一覧

    • 製品の詳細

      Mouse polyclonal to GOLPH3/MIDAS

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Recombinant full length protein within Human GOLPH3/MIDAS. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
      Database link: NP_071413

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab69171の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
    特記事項
    WB
    1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).

    ターゲット情報

    • 機能

      Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.

    • 組織特異性

      Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.

    • 配列類似性

      Belongs to the GOLPH3/VPS74 family.

    • 翻訳後修飾

      Phosphorylated.

    • 細胞内局在

      Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Coat protein antibody
        • Coat protein GPP34 antibody
        • FLJ90675 antibody
        • Golgi associated protein antibody
        • Golgi peripheral membrane protein 1, 34 kDa antibody
        • Golgi phosphoprotein 3 (coat protein) antibody
        • Golgi phosphoprotein 3 antibody
        • Golgi protein antibody
        • GOLP3_HUMAN antibody
        • Golph3 antibody
        • GOPP1 antibody
        • GPP34 antibody
        • MIDAS antibody
        • Mitochondrial DNA absence factor antibody
        see all

      画像

      • Western blot - Anti-GOLPH3/MIDAS antibody (ab69171)
        Western blot - Anti-GOLPH3/MIDAS antibody (ab69171)
        All lanes : Anti-GOLPH3/MIDAS antibody (ab69171) at 1/500 dilution

        Lane 1 : GOLPH3/MIDAS transfected 293T cell lysate
        Lane 2 : Non transfected 293T cell lysate

        Lysates/proteins at 25 µg per lane.

        Secondary
        All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

        Predicted band size: 34 kDa
        Observed band size: 30 kDa why is the actual band size different from the predicted?

      参考文献 (4)

      ab69171 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab69171 は 4 報の論文で使用されています。

      • Wang K  et al. GOLPH3 Promotes Cancer Growth by Interacting With STIP1 and Regulating Telomerase Activity in Pancreatic Ductal Adenocarcinoma. Front Oncol 10:575358 (2020). PubMed: 33134174
      • Chen S  et al. Widespread and Functional RNA Circularization in Localized Prostate Cancer. Cell 176:831-843.e22 (2019). PubMed: 30735634
      • Li H  et al. GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer. J Transl Med 10:168 (2012). WB . PubMed: 22905766
      • Wang JH  et al. High expression of GOLPH3 in esophageal squamous cell carcinoma correlates with poor prognosis. PLoS One 7:e45622 (2012). WB, IHC . PubMed: 23056210

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-6 of 6 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-GOLPH3/MIDAS antibody

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (RPE1 cell line)
      Permeabilization
      Yes - TritonX100
      Specification
      RPE1 cell line
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Mr. Hyunsoo Kim

      Verified customer

      投稿 Mar 10 2021

      Immunocytochemistry/ Immunofluorescence abreview for Anti-GOLPH3/MIDAS antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (RM-1 - mouse prostate cancer cells)
      Permeabilization
      Yes - 0.2% Triton X-100 in PBS
      Specification
      RM-1 - mouse prostate cancer cells
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Armen Petrosyan

      Verified customer

      投稿 Dec 03 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-GOLPH3/MIDAS antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (U2OS cells (Human Bone Osteosarcoma Epithelial Cel)
      Specification
      U2OS cells (Human Bone Osteosarcoma Epithelial Cel
      Blocking step
      (agent) for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Lawrence Welch

      Verified customer

      投稿 Jun 16 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-GOLPH3/MIDAS antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (Rat Hepatocytes)
      Permeabilization
      Yes - 0.2% Triton X-100 in PBS
      Specification
      Rat Hepatocytes
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Armen Petrosyan

      Verified customer

      投稿 Dec 21 2016

      Immunocytochemistry/ Immunofluorescence abreview for Anti-GOLPH3/MIDAS antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (KG1a)
      Permeabilization
      No
      Specification
      KG1a
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Armen Petrosyan

      Verified customer

      投稿 Mar 15 2012

      Question

      Testing discount code to test ab69171 in IF.

      Read More
      Answer

      DISCOUNT CODE: *********
      Expiration date: *********

      I am very pleased to hear you would like to accept our offer and test ab69171 in IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forIF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com